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Literature summary extracted from

  • Guo, Z.P.; Zhang, L.; Ding, Z.Y.; Wang, Z.X.; Shi, G.Y.
    Improving ethanol productivity by modification of glycolytic redox factor generation in glycerol-3-phosphate dehydrogenase mutants of an industrial ethanol yeast (2011), J. Ind. Microbiol. Biotechnol., 38, 935-943.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.8 biotechnology deletion of the NAD+-dependent glycerol-3-phosphate dehydrogenase gene in an industrial ethanol-producing strain and expression of either the non-phosphorylating NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase from Bacillus cereus, strain AG2A, or the NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase GAPDH from Kluyveromyces lactis, strain AG2B, in the deletion strain. Recombinant strain AG2A exhibits a 48.70% decrease in glycerol production and a 7.60% increase in ethanol yield relative to the amount of substrate consumed, while recombinant strain AG2B exhibits a 52.90% decrease in glycerol production and a 7.34% increase in ethanol yield relative to the amount of substrate consumed, compared with the wild-type strain. The maximum specific growth rates of the recombinant AG2A and AG2B are higher than that of the gpd2 deletion strain and are indistinguishable compared with the wild-type strain in anaerobic batch fermentations Saccharomyces cerevisiae
1.1.1.8 synthesis deletion of the NAD+-dependent glycerol-3-phosphate dehydrogenase gene in an industrial ethanol-producing strain and expression of either the non-phosphorylating NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase from Bacillus cereus, strain AG2A, or the NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase GAPDH from Kluyveromyces lactis, strain AG2B, in the deletion strain. Recombinant strain AG2A exhibits a 48.70% decrease in glycerol production and a 7.60% increase in ethanol yield relative to the amount of substrate consumed, while recombinant strain AG2B exhibits a 52.90% decrease in glycerol production and a 7.34% increase in ethanol yield relative to the amount of substrate consumed, compared with the wild-type strain. The maximum specific growth rates of the recombinant AG2A and AG2B are higher than that of the gpd2 deletion strain and are indistinguishable compared with the wild-type strain in anaerobic batch fermentations Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.9 expressed in Saccharomyces cerevisiae mutant AG2 with deletion of NAD+-dependent glycerol-3-phosphate dehydrogenase gene GPD2 Bacillus cereus
1.2.1.13 expressed in Saccharomyces cerevisiae mutant AG2 with deletion of NAD+-dependent glycerol-3-phosphate dehydrogenase gene GPD2 Kluyveromyces lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.9 D-glyceraldehyde 3-phosphate + NADP+ + H2O Bacillus cereus
-
 3-phospho-D-glycerate + NADPH + H+
-
 ?
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NADP+ Kluyveromyces lactis
-
 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Saccharomyces cerevisiae
-
-
-
1.2.1.9 Bacillus cereus
-
-
-
1.2.1.13 Kluyveromyces lactis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.9 D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
 Bacillus cereus 3-phospho-D-glycerate + NADPH + H+
-
 ?
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Kluyveromyces lactis 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.8 GDP2
-
 Saccharomyces cerevisiae
1.2.1.9 GAPN
-
 Bacillus cereus
1.2.1.9 non-phosphorylating NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase
-
 Bacillus cereus
1.2.1.13 NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase
-
 Kluyveromyces lactis
1.2.1.13 NADP+-GAPDH
-
 Kluyveromyces lactis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.9 NADP+
-
 Bacillus cereus
1.2.1.13 NADP+
-
 Kluyveromyces lactis