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Literature summary extracted from

  • Rodriguez-Martin, A.; Acosta, R.; Liddell, S.; Nunez, F.; Benito, M.J.; Asensio, M.A.
    Characterization of the novel antifungal chitosanase PgChP and the encoding gene from Penicillium chrysogenum (2010), Appl. Microbiol. Biotechnol., 88, 519-528.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 32000
-
 x * 32000, deduced from amino acid sequence Penicillium chrysogenum
3.2.1.132 37000
-
 x * 37000, two discrete bands appear in SDS-PAGE: an intensely stained band showing a molecular mass of about 37 kDa and a fainter band of around 40 kDa Penicillium chrysogenum
3.2.1.132 40000
-
 x * 40000, two discrete bands appear in SDS-PAGE: an intensely stained band showing a molecular mass of about 37000 Da and a fainter band of around 40000 Da Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Penicillium chrysogenum D7R809 chitosanase preprotein
-
3.2.1.132 Penicillium chrysogenum AS51D D7R809 chitosanase preprotein
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.132 glycoprotein
-
 Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O the enzyme liberates a mean of 0.6 mM D-glucosamine per microgram of protein in 4 h reaction Penicillium chrysogenum ?
-
 ?
3.2.1.132 chitosan + H2O the enzyme liberates a mean of 0.6 mM D-glucosamine per microgram of protein in 4 h reaction Penicillium chrysogenum AS51D ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 30000-31000, analysis of the molecular mass by electrospray ionization-mass spectrometry reveals six major peaks from 30000 to 31000 Da that are related to different levels of glycosylation. The mass profile shows a dominant peak of 30772 Da with a series of surrounding peaks differing from each other by 162 Da Penicillium chrysogenum
3.2.1.132 ? x * 32000, deduced from amino acid sequence Penicillium chrysogenum
3.2.1.132 ? x * 37000, two discrete bands appear in SDS-PAGE: an intensely stained band showing a molecular mass of about 37 kDa and a fainter band of around 40 kDa Penicillium chrysogenum
3.2.1.132 ? x * 40000, two discrete bands appear in SDS-PAGE: an intensely stained band showing a molecular mass of about 37000 Da and a fainter band of around 40000 Da Penicillium chrysogenum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.132 PgChP
-
 Penicillium chrysogenum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.132 Penicillium chrysogenum isoelectric focusing
-
 5