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Literature summary extracted from

  • Chao, L.Y.; Rine, J.; Marletta, M.A.
    Spectroscopic and kinetic studies of Nor1, a cytochrome P450 nitric oxide reductase from the fungal pathogen Histoplasma capsulatum (2008), Arch. Biochem. Biophys., 480, 132-137.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.14 a 404 amino acid fragment of Nor1p is expressed in Escherichia coli JM109 cells Histoplasma capsulatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.14 0.02
-
 NO in 100 mM potassium phosphate (pH 7.5), at 25°C Histoplasma capsulatum

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.14 Histoplasma capsulatum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.14 Ni-NTA column chromatography and Poros HQ anion-exchange column chromatography Histoplasma capsulatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.14 DEA-NONOate + NADH + H+
-
 Histoplasma capsulatum N2O + NAD+ + ?
-
 ?
1.7.1.14 NO + NADH + H+
-
 Histoplasma capsulatum N2O + NAD+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.14 cytochrome P450 nitric oxide reductase
-
 Histoplasma capsulatum
1.7.1.14 Nor1p
-
 Histoplasma capsulatum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.1.14 83.3
-
 NO in 100 mM potassium phosphate (pH 7.5), at 25°C Histoplasma capsulatum

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.14 heme
-
 Histoplasma capsulatum
1.7.1.14 NADH Nor1p oxidizes NADH but has no activity with NADPH Histoplasma capsulatum

General Information

EC Number General Information Comment Organism
1.7.1.14 physiological function Nor1p plays a protective role against macrophage-derived nitric oxide Histoplasma capsulatum