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Literature summary extracted from

  • Minghetti, K.C.; Goswitz, V.C.; Gabriel, N.E.; Hill, J.J.; Barassi, C.A.; Georgiou, C.D.; Chan, S.I.; Gennis, R.B.
    Modified, large-scale purification of the cytochrome o complex (bo-type oxidase) of Escherichia coli yields a two heme/one copper terminal oxidase with high specific activity (1992), Biochemistry, 31, 6917-6924.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.3 membrane
-
 Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.3 Cu2+ the pure four-subunit enzyme contains one equivalent of copper (7.5 ng/mg) Escherichia coli
7.1.1.3 Fe the pure four-subunit enzyme contains two equivalents of iron (19.5 ng/mg) Escherichia coli
7.1.1.3 additional information the enzyme contains no zinc. The equivalent of CuA of the aa3-type cytochrome c oxidases is absent in this quinol oxidase Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.3 17000
-
 1 * 58000 + 1 * 33000 + 1 * 22000 + 1 * 17000, SDS-PAGE Escherichia coli
7.1.1.3 22000
-
 1 * 58000 + 1 * 33000 + 1 * 22000 + 1 * 17000, SDS-PAGE Escherichia coli
7.1.1.3 33000
-
 1 * 58000 + 1 * 33000 + 1 * 22000 + 1 * 17000, SDS-PAGE Escherichia coli
7.1.1.3 58000
-
 1 * 58000 + 1 * 33000 + 1 * 22000 + 1 * 17000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.3 Escherichia coli
-
-
-
7.1.1.3 Escherichia coli RG145
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.3 DEAE-Sepharose column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1.1.3 960
-
 at 37°C, pH not specified in the publication Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.3 additional information the enzyme does not interact with cytochrome c Escherichia coli ?
-
 ?
7.1.1.3 additional information the enzyme does not interact with cytochrome c Escherichia coli RG145 ?
-
 ?
7.1.1.3 ubiquinol-1 + O2 + H+/in
-
 Escherichia coli ubiquinone-1 + H2O + H+/out
-
 ?
7.1.1.3 ubiquinol-1 + O2 + H+/in
-
 Escherichia coli RG145 ubiquinone-1 + H2O + H+/out
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.3 heterotetramer 1 * 58000 + 1 * 33000 + 1 * 22000 + 1 * 17000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
7.1.1.3 bo-type ubiquinol oxidase
-
 Escherichia coli
7.1.1.3 cytochrome o complex
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.3 heme the enzyme possesses two hemes (18.1 ng/mg) Escherichia coli