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Literature summary extracted from
- Mapping the intestinal alpha-glucogenic enzyme specificities of starch digesting maltase-glucoamylase and sucrase-isomaltase (2011), Bioorg. Med. Chem., 19, 3929-3934.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.10 | recombinant expression of N-terminally His-tagged enzyme in Drosophila melanogaster S2 cells | Homo sapiens |
| 3.2.1.20 | N- and C-terminal catalytic subunits, expression of His6-tagged N-terminal subunit ntMGAM in Drosophila S2 cells, expression of His6-tagged C-terminal subunits ctMGAMN2 and ctMGAMN20 in Spodoptera frugiperda Sf9 cells | Homo sapiens |
| 3.2.1.20 | recombinant expression of N-terminally His-tagged N-terminal domain in Drosophila melanogaster S2 cells | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.10 | acarbose | - |
Homo sapiens |  |
| 3.2.1.10 | blintol | selenium analogue of salacinol | Homo sapiens | |
| 3.2.1.10 | de-O-sulfonated kotalanol | isolated from Salacia reticulata | Homo sapiens | |
| 3.2.1.10 | kotalanol | isolated from Salacia reticulata | Homo sapiens | |
| 3.2.1.10 | miglitol | - |
Homo sapiens | |
| 3.2.1.10 | additional information | inhibition profiles of the individual N- and C-terminal catalytic subunits of the enzyme by clinical glucosidase inhibitors, acarbose and miglitol, and glucosidase inhibitors from an Ayurvedic remedy used for the treatment of type II diabetes, overview | Homo sapiens | |
| 3.2.1.10 | salacinol | isolated from Salacia reticulata | Homo sapiens | |
| 3.2.1.20 | acarbose | - |
Homo sapiens | |
| 3.2.1.20 | blintol | selenium analogue of salacinol | Homo sapiens | |
| 3.2.1.20 | de-O-sulfonated kotalanol | isolated from Salacia reticulata | Homo sapiens | |
| 3.2.1.20 | kotalanol | isolated from Salacia reticulata | Homo sapiens | |
| 3.2.1.20 | maltotriose | exhibits strong substrate inhibition; strong substrate inhibition | Homo sapiens | |
| 3.2.1.20 | miglitol | - |
Homo sapiens | |
| 3.2.1.20 | additional information | inhibition profiles of the individual N- and C-terminal catalytic subunits of the enzyme by clinical glucosidase inhibitors, acarbose and miglitol, and glucosidase inhibitors from an Ayurvedic remedy used for the treatment of type II diabetes, overview; inhibition profiles of the individual N- and C-terminal catalytic subunits of the enzyme, overview | Homo sapiens | |
| 3.2.1.20 | salacinol | isolated from Salacia reticulata | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.20 | 1.59 | - |
maltose | catalytic subunit ctMGAMN2, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 1.91 | - |
maltose | catalytic subunit ctMGAMN20, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 4.3 | - |
maltose | catalytic subunit ntMGAM, pH and temperature not specified in the publication | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.20 | maltose + H2O | Homo sapiens | - |
2 D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.10 | Homo sapiens | P14410 | - |
- |
| 3.2.1.20 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.10 | recombinant N-terminally His-tagged enzyme from Drosophila melanogaster S2 cells by copper affinity chromatography and ion exchange chromatography | Homo sapiens |
| 3.2.1.20 | recombinant His6-tagged C-terminal subunits ctMGAMN2 and ctMGAMN20 from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography, recombinant His6-tagged N-terminal subunit ntMGAM from Drosophila S2 by cobalt affinity chromatography and ion exchange chromatography | Homo sapiens |
| 3.2.1.20 | recombinant N-terminally His-tagged N-terminal domain from Drosophila melanogaster S2 cells by copper affinity chromatography and ion exchange chromatography | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.10 | small intestine | - |
Homo sapiens | - |
| 3.2.1.20 | intestine | - |
Homo sapiens | - |
| 3.2.1.20 | small intestine | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.10 | additional information | the enzyme is active with maltose, maltotriose, and sucrose, only the N-terminal catalytic domain acts as alpha-1,6-glucosidase | Homo sapiens | ? | - |
? | |
| 3.2.1.20 | maltose + H2O | - |
Homo sapiens | 2 D-glucose | - |
? | |
| 3.2.1.20 | additional information | the enzyme is active with maltose, maltotriose, and sucrose | Homo sapiens | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.10 | More | the enzyme belongs to the family 31 of glycoside hydrolases | Homo sapiens |
| 3.2.1.10 | sucrase-isomaltase | - |
Homo sapiens |
| 3.2.1.20 | maltase-glucoamylase | - |
Homo sapiens |
| 3.2.1.20 | MGAM | - |
Homo sapiens |
| 3.2.1.20 | More | the enzyme is a family 31 glycoside hydrolase | Homo sapiens |
| 3.2.1.20 | More | the enzyme belongs to the family 31 of glycoside hydrolases | Homo sapiens |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.10 | 0.000012 | - |
de-O-sulfonated kotalanol | N-terminal cataklytic domain, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.10 | 0.000148 | - |
miglitol | N-terminal cataklytic domain, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.10 | 0.00016 | - |
blintol | N-terminal cataklytic domain, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.10 | 0.000277 | - |
salacinol | N-terminal cataklytic domain, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.10 | 0.0006 | - |
kotalanol | N-terminal cataklytic domain, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.10 | 0.014 | - |
acarbose | N-terminal cataklytic domain, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000009 | - |
acarbose | C-terminal catalytic domain N2, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000009 | - |
acarbose | catalytic subunit ctMGAMN2, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000013 | - |
blintol | C-terminal catalytic domain N20, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000013 | - |
blintol | catalytic subunit ctMGAMN20, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000018 | - |
blintol | C-terminal catalytic domain N2, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000018 | - |
blintol | catalytic subunit ctMGAMN2, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000026 | - |
de-O-sulfonated kotalanol | C-terminal catalytic domain N2, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000026 | - |
de-O-sulfonated kotalanol | catalytic subunit ctMGAMN2, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000028 | - |
acarbose | C-terminal catalytic domain N20, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000028 | - |
acarbose | catalytic subunit ctMGAMN20, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.00003 | - |
de-O-sulfonated kotalanol | catalytic subunit ntMGAM, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.00003 | - |
de-O-sulfonated kotalanol | N-terminal catalytic domain, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000058 | - |
salacinol | C-terminal catalytic domain N20, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000058 | - |
salacinol | catalytic subunit ctMGAMN20, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000064 | - |
kotalanol | C-terminal catalytic domain N2, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000064 | - |
kotalanol | catalytic subunit ctMGAMN2, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000078 | - |
de-O-sulfonated kotalanol | C-terminal catalytic domain N20, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000078 | - |
de-O-sulfonated kotalanol | catalytic subunit ctMGAMN20, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000092 | - |
kotalanol | C-terminal catalytic domain N20, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000092 | - |
kotalanol | catalytic subunit ctMGAMN20, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.00019 | - |
salacinol | catalytic subunit ntMGAM, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.00019 | - |
kotalanol | catalytic subunit ntMGAM, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.00019 | - |
salacinol | N-terminal catalytic domain, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.00019 | - |
kotalanol | N-terminal catalytic domain, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000211 | - |
miglitol | C-terminal catalytic domain N2, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000211 | - |
miglitol | catalytic subunit ctMGAMN2, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000213 | - |
salacinol | C-terminal catalytic domain N2, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.000213 | - |
salacinol | catalytic subunit ctMGAMN2, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.00023 | - |
miglitol | C-terminal catalytic domain N20, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.00023 | - |
miglitol | catalytic subunit ctMGAMN20, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.00049 | - |
blintol | catalytic subunit ntMGAM, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.00049 | - |
blintol | N-terminal catalytic domain, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.001 | - |
miglitol | catalytic subunit ntMGAM, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.001 | - |
miglitol | N-terminal catalytic domain, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.062 | - |
acarbose | catalytic subunit ntMGAM, pH and temperature not specified in the publication | Homo sapiens | |
| 3.2.1.20 | 0.062 | - |
acarbose | N-terminal catalytic domain, pH and temperature not specified in the publication | Homo sapiens | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.20 | metabolism | the enzyme is responsible for the final step of starch hydrolysis | Homo sapiens |
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