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Literature summary extracted from
- Structurally informed site-directed mutagenesis of a stereochemically promiscuous aldolase to afford stereochemically complementary biocatalysts (2010), J. Am. Chem. Soc., 132, 11753-11758.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.2.55 | expression in Escherichia coli | Saccharolobus solfataricus |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.1.2.55 | X-ray crystallographic analysis of the active site of the aldolase bound to pyruvate, 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate at 4°C, reveals that its catalytic mechanism is typical of the N-acetylneuraminic acid lyase superfamily | Saccharolobus solfataricus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.1.2.55 | T157C | improved for 2-dehydro-3-deoxy-D-gluconate with a 75% diastereoisomeric ratio | Saccharolobus solfataricus |
| 4.1.2.55 | T157C/Y132V | diastereoselective formation of 2-dehydro-3-deoxy-D-gluconate with much improved (91%) diastereoisomeric ratio | Saccharolobus solfataricus |
| 4.1.2.55 | T157F/Y132V | diastereoselective formation of 2-dehydro-3-deoxy-D-gluconate with much improved (93%) diastereoisomeric ratio | Saccharolobus solfataricus |
| 4.1.2.55 | T157V/A198L/D181Q | diastereoselective formation of 2-dehydro-3-deoxy-D-galactonate with much improved (88%) diastereoisomeric ratio | Saccharolobus solfataricus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.55 | 1.1 | - |
pyruvate | 70°C, pH is not specified in the publication, wild-type enzyme | Saccharolobus solfataricus |  |
| 4.1.2.55 | 4.1 | - |
pyruvate | 70°C, pH is not specified in the publication, mutant enzyme T157F/Y132V | Saccharolobus solfataricus | |
| 4.1.2.55 | 5.3 | - |
D-glyceraldehyde | 70°C, pH is not specified in the publication, wild-type enzyme T157F/Y132V | Saccharolobus solfataricus | |
| 4.1.2.55 | 8 | - |
D-glyceraldehyde | 70°C, pH is not specified in the publication, mutant enzyme | Saccharolobus solfataricus | |
| 4.1.2.55 | 9.1 | - |
D-glyceraldehyde | 70°C, pH is not specified in the publication, mutant enzyme T157C/Y132V | Saccharolobus solfataricus | |
| 4.1.2.55 | 39.6 | - |
pyruvate | 70°C, pH is not specified in the publication, mutant enzyme T157C/Y132V | Saccharolobus solfataricus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.2.55 | Saccharolobus solfataricus | O54288 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.2.55 | - |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.55 | pyruvate + D-glyceraldehyde | the enzyme exhibits poor diastereocontrol in many of its aldol reactions, including the reaction of its natural substrates, pyruvate and D-glyceraldehyde, which afford a 55:45 mixture of D-2-keto-3-deoxygluconate and D-2-keto-3-deoxy-galactonate | Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-galactonate | - |
r | |
| 4.1.2.55 | pyruvate + D-glyceraldehyde | the enzyme exhibits poor diastereocontrol in many of its aldol reactions, including the reaction of its natural substrates, pyruvate and D-glyceraldehyde, which afford a 55:45 mixture of D-2-keto-3-deoxygluconate and D-2-keto-3-deoxy-galactonate | Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-gluconate | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.2.55 | 2-keto-3-deoxygluconate aldolase | - |
Saccharolobus solfataricus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.55 | 0.06 | - |
D-glyceraldehyde | 70°C, pH is not specified in the publication, mutant enzyme | Saccharolobus solfataricus | |
| 4.1.2.55 | 0.07 | - |
pyruvate | 70°C, pH is not specified in the publication, mutant enzyme T157C/Y132V | Saccharolobus solfataricus | |
| 4.1.2.55 | 0.07 | - |
D-glyceraldehyde | 70°C, pH is not specified in the publication, mutant enzyme T157C/Y132V | Saccharolobus solfataricus | |
| 4.1.2.55 | 0.075 | - |
pyruvate | 70°C, pH is not specified in the publication, mutant enzyme T157F/Y132V | Saccharolobus solfataricus | |
| 4.1.2.55 | 4.6 | - |
pyruvate | 70°C, pH is not specified in the publication, wild-type enzyme | Saccharolobus solfataricus | |
| 4.1.2.55 | 5.5 | - |
D-glyceraldehyde | 70°C, pH is not specified in the publication, wild-type enzyme T157F/Y132V | Saccharolobus solfataricus | |
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