EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.57 | expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.57 | 2,2'-dipyridyl | 2 mM 2,2'-dipyridyl, a specific Fe2+-chelator, abolishes the gallate dioxygenase activity completely | Pseudomonas putida | |
1.13.11.57 | EDTA | complete inhibition at 1 mM, the activity is only fully recovered with Fe2+ | Pseudomonas putida | |
1.13.11.57 | H2O2 | hydrogen peroxide at 1 mM reduces the gallate dioxygenase activity to 15%, and the activity can be partially restored (50% activity of the untreated enzyme) upon addition of 10 mM ascorbate | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.57 | 0.144 | - |
gallate | in 100 mM Tris-HCl, pH 7.0, at 30°C | Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.57 | Fe2+ | required for activity | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.57 | 47000 | - |
3 * 47000, SDS-PAGE | Pseudomonas putida |
1.13.11.57 | 47644 | - |
3 * 47644, calculated from amino acid sequence | Pseudomonas putida |
1.13.11.57 | 132000 | - |
molar mass obtained by sedimentation equilibrium | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.57 | Pseudomonas putida | - |
- |
- |
1.13.11.57 | Pseudomonas putida KT 2240 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.57 | ultracentrifugation and phenyl-Sepharose column chromatography | Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.57 | 21.4 | - |
crude extract, in 100 mM Tris-HCl, pH 7.0, at 30°C | Pseudomonas putida |
1.13.11.57 | 42.7 | - |
after 2fold purification, in 100 mM Tris-HCl, pH 7.0, at 30°C | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.57 | gallate + O2 | the substrate specificity is very restricted toward gallate | Pseudomonas putida | 4-oxalomesaconate | - |
? | |
1.13.11.57 | gallate + O2 | the substrate specificity is very restricted toward gallate | Pseudomonas putida KT 2240 | 4-oxalomesaconate | - |
? | |
1.13.11.57 | additional information | no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate | Pseudomonas putida | ? | - |
? | |
1.13.11.57 | additional information | no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate | Pseudomonas putida KT 2240 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.57 | homotrimer | 3 * 47000, SDS-PAGE | Pseudomonas putida |
1.13.11.57 | homotrimer | 3 * 47644, calculated from amino acid sequence | Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.57 | GALA | - |
Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.57 | 7 | - |
- |
Pseudomonas putida |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.57 | 6 | 9 | - |
Pseudomonas putida |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.13.11.57 | Pseudomonas putida | the expression of the galA gene is specifically induced by gallate | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.11.57 | physiological function | the GalA protein is the only dioxygenase involved in gallate cleavage in Pseudomonas putida KT2440 | Pseudomonas putida |