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Literature summary extracted from

  • Yi, S.M.; Narasimhulu, K.V.; Samoilova, R.I.; Gennis, R.B.; Dikanov, S.A.
    Characterization of the semiquinone radical stabilized by the cytochrome aa3-600 menaquinol oxidase of Bacillus subtilis (2010), J. Biol. Chem., 285, 18241-18251.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.5 expressed in Bacillus subtilis strain LUW143 lacking both the aa3-600 menaquinol oxidase and caa3-type cytochrome c oxidase Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.5 Cu2+ cytochrome aa3-600 is a member of the heme-copper superfamily of oxygen reductases Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.5 menaquinol + O2 Bacillus subtilis cyt aa3-600 is strictly a menaquinol oxidase menaquinone + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.5 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.5 Ni-NTA column chromatography, 1.25 equivalents of menaquinone-7 co-purify with the enzyme Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.5 2 2,3-dimethyl-1,4-naphthoquinol + O2 + n H+[side 1]
-
 Bacillus subtilis 2 2,3-dimethyl-1,4-naphthoquinone + 2 H2O + n H+[side 2]
-
 ?
7.1.1.5 menaquinol + O2 cyt aa3-600 is strictly a menaquinol oxidase Bacillus subtilis menaquinone + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.5 cyt aa3-600
-
 Bacillus subtilis
7.1.1.5 cytochrome aa3-600 menaquinol oxidase
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.5 61
-
 2,3-dimethyl-1,4-naphthoquinone in 50 mM Tris, pH 7.0, at 25°C Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.5 heme cytochrome aa3-600 is a member of the heme-copper superfamily of oxygen reductases Bacillus subtilis