EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.99.2 | expressed in Escherichia coli BL21(DE3)R3-Rosetta cells | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.4.99.2 | enzyme in the apo, holo, and substrate-bound ternary forms, sitting drop vapor diffusion method, using 1.6 M Na/K-phosphate, 0.1 M HEPES pH 7.5 (apo form), or 30% (w/v) PEG3350, 0.1 M Bis-Tris pH 5.5, 0.3 M (NH4)2SO4 (holo form), or 20% (w/v) PEG3350, 0.1 M Bis-Tris pH 5.5, 0.1 M (NH4)2SO4 (ternary form) | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
5.4.99.2 | mitochondrion | - |
Homo sapiens | 5739 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.2 | Homo sapiens | P22033 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.99.2 | Ni-NTA column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.2 | (R)-2-methylmalonyl-CoA | - |
Homo sapiens | succinyl-CoA | - |
? | |
5.4.99.2 | additional information | human MMAA and MUT interact in vitro and in vivo. This interaction is nucleotide-selective for MMAA and apoenzyme-dependent for MUT and is associated with stimulation of MMAA GTPase activity by MUT | Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.4.99.2 | homodimer | x-ray crystallography | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.2 | MCM | - |
Homo sapiens |
5.4.99.2 | MuT | - |
Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.2 | adenosylcobalamin | - |
Homo sapiens | |
5.4.99.2 | Cobalamin | essential to the function of methylmalonyl-CoA mutase, cobalamin is conversed to adenosylcobalamin | Homo sapiens |