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Literature summary extracted from

  • Jin, Q.; Yuan, X.; Cui, W.; Han, B.; Feng, J.; Xu, S.; Shen, W.
    Isolation and characterization of a heme oxygenase-1 gene from Chinese cabbage (2012), Mol. Biotechnol., 50, 8-17.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.18 DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic analysis, functional expression of His-tagged HO1 in Escherichia coli Brassica rapa subsp. pekinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.18 additional information
-
 additional information Michaelis-Menten kinetics, overview Brassica rapa subsp. pekinensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.18 chloroplast
-
 Brassica rapa subsp. pekinensis 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.18 heme + 3 AH2 + 3 O2 Brassica rapa subsp. pekinensis
-
 biliverdin IXalpha + Fe2+ + CO + 3 A + 3 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.18 Brassica rapa subsp. pekinensis H8XZ68 two types of isoforms: an inducible heme oxygenase-1, HO1, and a constitutive heme oxygenase-2/3, HO2/3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.18 recombinant His-tagged HO1 from Escherichia coli by nickel affinity chromatography Brassica rapa subsp. pekinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.18 leaf young Brassica rapa subsp. pekinensis
-
1.14.14.18 root
-
 Brassica rapa subsp. pekinensis
-
1.14.14.18 seed germinating Brassica rapa subsp. pekinensis
-
1.14.14.18 seedling
-
 Brassica rapa subsp. pekinensis
-
1.14.14.18 stem
-
 Brassica rapa subsp. pekinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.18 heme + 3 AH2 + 3 O2
-
 Brassica rapa subsp. pekinensis biliverdin IXalpha + Fe2+ + CO + 3 A + 3 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.18 More three-dimensional structure of BrHO1, analysis and comparison, overview Brassica rapa subsp. pekinensis

Synonyms

EC Number Synonyms Comment Organism
1.14.14.18 heme oxygenase-1
-
 Brassica rapa subsp. pekinensis
1.14.14.18 Ho1
-
 Brassica rapa subsp. pekinensis

Expression

EC Number Organism Comment Expression
1.14.14.18 Brassica rapa subsp. pekinensis HO1 is induced by several stress conditions, e.g. osmotic and salinity stresses, cadmium exposure, H2O2, and hemin treatments up

General Information

EC Number General Information Comment Organism
1.14.14.18 physiological function BrHO1 may play an important role in abiotic stress tolerance of Chinese cabbage. Heme oxygenase is a regulatory enzyme that cleaves heme to biliverdin IXalpha, with the concomitant release of carbon monoxide and the production of free Fe2+ Brassica rapa subsp. pekinensis