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Literature summary extracted from

  • Yadav, S.; Bisht, D.; Shikh, S.; Darmwal, N.
    Oxidant and solvent stable alkaline protease from Aspergillus flavus and its characterization (2011), Afr. J. Biotechnol., 10, 8630-8640.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.4.21.63 industry possible use of the enzyme in detergent industry and peptide synthesis due to its compatibility with several detergents, oxidants and organic solvents Aspergillus flavus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.63 Co2+ about 10% inhibition at 5 mM Aspergillus flavus
3.4.21.63 Cu2+ over 90% inhibition at 5 mM Aspergillus flavus
3.4.21.63 Hg2+ complete inhibition at 5 mM Aspergillus flavus
3.4.21.63 Mn2+ about 30% inhibition at 5 mM Aspergillus flavus
3.4.21.63 additional information poor inhibition by Ca2+ at 5 mM Aspergillus flavus
3.4.21.63 Ni2+ about 20% inhibition at 5 mM Aspergillus flavus
3.4.21.63 Zn2+ about 20% inhibition at 5 mM Aspergillus flavus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.63 extracellular
-
 Aspergillus flavus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.63 Fe2+ activates 1.74fold at 5 mM Aspergillus flavus
3.4.21.63 K+ activates about 10% at 5 mM Aspergillus flavus
3.4.21.63 additional information no effect on enzyme activity by 5 mM Pb2+. Effect of metal salts on enzyme production, overview Aspergillus flavus
3.4.21.63 Na+ activates about 10% at 5 mM Aspergillus flavus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.21.63 Acetone 97.1% remaining activity after 1 h at 30°C in 25% organic solvent Aspergillus flavus
3.4.21.63 benzene 89.2% remaining activity after 1 h at 30°C in 25% organic solvent Aspergillus flavus
3.4.21.63 Butanol 96.2% remaining activity after 1 h at 30°C in 25% organic solvent Aspergillus flavus
3.4.21.63 chloroform 105.3% activity after 1 h at 30°C in 25% organic solvent Aspergillus flavus
3.4.21.63 diethyl ether 89.2% remaining activity after 1 h at 30°C in 25% organic solvent Aspergillus flavus
3.4.21.63 Ethanol 83.2% remaining activity after 1 h at 30°C in 25% organic solvent Aspergillus flavus
3.4.21.63 hexane 94.3% remaining activity after 1 h at 30°C in 25% organic solvent Aspergillus flavus
3.4.21.63 Methanol 81.7% remaining activity after 1 h at 30°C in 25% organic solvent Aspergillus flavus
3.4.21.63 additional information extremely organic solvent stable serine protease. The enzyme retains 80% of its original activity in the presence of non ionic and ionic surfactants after 1 h of incubation at 30°C, overview Aspergillus flavus
3.4.21.63 toluene 91.7% remaining activity after 1 h at 30°C in 25% organic solvent Aspergillus flavus
3.4.21.63 xylene 83.6% remaining activity after 1 h at 30°C in 25% organic solvent Aspergillus flavus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.63 Aspergillus flavus
-
-
-
3.4.21.63 Aspergillus flavus MTCC 9952
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.4.21.63 oxidant stable serine protease. The enzyme retains 100% of its original activity with 10% H2O2 after 1 h of incubation at 30°C Aspergillus flavus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.63 native enzyme partially by acetone precipitation Aspergillus flavus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.63 additional information the organism is grown under solid state fermentation. Maximum protease yield is obtained when the strain is grown under wheat bran and corn cob mixture, ratio 1:1, incubated for 48 h at pH 9.0 and temperature 37°C with 50% of initial moisture content, evaluation overview Aspergillus flavus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.63 casein + H2O artificial substrate Aspergillus flavus ?
-
 ?
3.4.21.63 casein + H2O artificial substrate Aspergillus flavus MTCC 9952 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.63 40
-
-
 Aspergillus flavus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.63 30 55 100% activity at 40°C while 92% residual activity at 45°C, 74% at 50°C, and 41% 55°C, inactive above 55°C, temperature profile, overview Aspergillus flavus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.63 additional information
-
 thermal stability profile, overview Aspergillus flavus
3.4.21.63 30 40 partially purified enzyme, stable at, retention of 96% activity after 1 h of incubation at 40°C Aspergillus flavus
3.4.21.63 45
-
 84% residual activity after 1 h of incubation at 45°C Aspergillus flavus
3.4.21.63 55
-
 53% residual activity after 1 h of incubation at 55°C Aspergillus flavus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.63 8 12
-
 Aspergillus flavus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.63 7 12 partially purified enzyme, stable at Aspergillus flavus

General Information

EC Number General Information Comment Organism
3.4.21.63 additional information the enzyme retains 80% of its original activity in the presence of non ionic and ionic surfactants after 1 h of incubation at 30°C, overview Aspergillus flavus