EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.245 | methylcob(III)-amide | product inhibition | Moorella thermoacetica | |
2.1.1.245 | tetrahydrofolate | product inhibition | Moorella thermoacetica | |
2.1.1.258 | tetrahydrofolate | competitive inhibitor | Moorella thermoacetica | |
2.1.1.258 | [methyl-Co(III) corrinoid Fe-S protein] | competitive inhibitor | Moorella thermoacetica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.245 | additional information | - |
additional information | single-turnover stopped-flow studies, steady-state and pre-steady-state kinetic studies at different pH values and by kinetic simulations, random Bi-Bi reaction mechanism, detailed overview | Moorella thermoacetica | |
2.1.1.245 | 0.002 | - |
tetrahydrosarcinapterin | pH 7.6, 25°C | Moorella thermoacetica | |
2.1.1.245 | 0.012 | - |
a [methyl-Co(III) corrinoid Fe-S protein] | pH 7.6, 25°C | Moorella thermoacetica | |
2.1.1.258 | 0.002 | - |
5-methyltetrahydrofolate | in 50 mM MES, pH 5.1, at 25°C | Moorella thermoacetica | |
2.1.1.258 | 0.012 | - |
[Co(I) corrinoid Fe-S protein] | in 50 mM MES, pH 5.1, at 25°C | Moorella thermoacetica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.245 | a [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrosarcinapterin | Moorella thermoacetica | a pH-dependent conformational change is required for methyl transfer in the forward and reverse directions, but this step is not rate-limiting | a [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrosarcinapterin | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.245 | Moorella thermoacetica | - |
- |
- |
2.1.1.258 | Moorella thermoacetica | - |
formerly Clostridium thermoaceticum | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.258 | - |
Moorella thermoacetica |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.1.1.245 | a [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrosarcinapterin = a [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrosarcinapterin | random Bi-Bi mechanism of transfer of the methyl group from (6S)-methyltetrahydrofolate to the corrinoid/iron-sulfur protein: CH3-H4folate binds to MeTr in the unprotonated form and then undergoes rapid protonation. This protonation enhances the electrophilicity of the methyl group, in agreement with a 10fold increase in the pKa at N5 of CH3-H4folate. Next, the Co(I)-CFeSP attacks the methyl group in a rate-limiting SN2 reaction to form methylcob(III)amide. Finally, the products randomly dissociate. A pH-dependent conformational change is required for methyl transfer in the forward and reverse directions, a rate-limiting ionization of MeTr, not of CH3-H4folate, is responsible for the pH dependence of the methyl transfer reaction | Moorella thermoacetica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.245 | a [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrosarcinapterin | a pH-dependent conformational change is required for methyl transfer in the forward and reverse directions, but this step is not rate-limiting | Moorella thermoacetica | a [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrosarcinapterin | - |
r | |
2.1.1.245 | a [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrosarcinapterin | a pH-dependent conformational change is required for methyl transfer in the forward and reverse directions, but this step is not rate-limiting, the pH-dependent protein conformational change in the ternary complex is linked to deprotonation of the active complexes | Moorella thermoacetica | a [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrosarcinapterin | - |
r | |
2.1.1.245 | additional information | the methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase catalyzes transfer of the N5-methyl group from (6S)-methyltetrahydrofolate, i.e. CH3-H4folate, to the cobalt center of a corrinoid/iron-sulfur protein, CFeSP, forming methylcob(III)-amide and H4folate | Moorella thermoacetica | ? | - |
? | |
2.1.1.258 | [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate | - |
Moorella thermoacetica | [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.245 | methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase | - |
Moorella thermoacetica |
2.1.1.245 | MeTr | - |
Moorella thermoacetica |
2.1.1.258 | methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase | - |
Moorella thermoacetica |
2.1.1.258 | MeTr | - |
Moorella thermoacetica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.245 | 25 | - |
assay at | Moorella thermoacetica |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.245 | 14.7 | - |
tetrahydrosarcinapterin | pH 7.6, 25°C | Moorella thermoacetica | |
2.1.1.245 | 14.7 | - |
a [methyl-Co(III) corrinoid Fe-S protein] | pH 7.6, 25°C | Moorella thermoacetica | |
2.1.1.258 | 14.7 | - |
5-methyltetrahydrofolate | in 50 mM MES, pH 5.1, at 25°C | Moorella thermoacetica | |
2.1.1.258 | 14.7 | - |
[Co(I) corrinoid Fe-S protein] | in 50 mM MES, pH 5.1, at 25°C | Moorella thermoacetica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.245 | 7.6 | - |
assay at | Moorella thermoacetica |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.245 | metabolism | the enzyme reaction initiates the unusual biological organometallic reaction sequence that constitutes the Wood-Ljungdahl or reductive acetyl-CoA pathway | Moorella thermoacetica |
2.1.1.258 | metabolism | the enzyme performs a key step in the Wood-Ljungdahl pathway of acetyl-CoA synthesis | Moorella thermoacetica |