EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.18 | expression of His-tagged viral neuraminidase in wild-type Pichia pastoris strain GS115, an alpha-1,6-mannosyltransferase (och1)-defective Pichia pastoris mutant strain, and in Escherichia coli, resulting in a hyper-glycosylated, a low-glycosylated, and a non-glycosylated enzyme, respectively | influenza A virus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.18 | 45000 | - |
x * 59000-97000, hyperglycosylated recombinant enzyme, SDS-PAGE, x * 66000, low-glycosylated recombinant enzyme, SDS-PAGE, x * 45000, non-glycosylated recombinant enzyme, SDS-PAGE | influenza A virus |
3.2.1.18 | 66000 | - |
x * 59000-97000, hyperglycosylated recombinant enzyme, SDS-PAGE, x * 66000, low-glycosylated recombinant enzyme, SDS-PAGE, x * 45000, non-glycosylated recombinant enzyme, SDS-PAGE | influenza A virus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.18 | influenza A virus | Q7TG96 | - |
- |
3.2.1.18 | influenza A virus newCaledonia/20/99(H1N1) | Q7TG96 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.18 | recombinant His-tagged viral neuraminidase by nickel affinity chromatography, desalting gel filtration, and cation exchange chromatography | influenza A virus |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.18 | ? | x * 59000-97000, hyperglycosylated recombinant enzyme, SDS-PAGE, x * 66000, low-glycosylated recombinant enzyme, SDS-PAGE, x * 45000, non-glycosylated recombinant enzyme, SDS-PAGE | influenza A virus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.18 | neuraminidase | - |
influenza A virus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.18 | additional information | low-glycosylation in och1-defective Pichia pastoris enhances the immunogenicity of recombinant neuraminidase and elicits similar antibody titers with less antigen when compared with hyper- and non-glycosylated neuraminidases after vaccination of mice | influenza A virus |