EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.164 | gene penDE, lack of correlation between the protein and transcript levels of the IAT enzyme, quantitative real-time PCR expression analysis and gene copy number determination, overexpression of penDE in Penicillium chrysogenum strains DS47274, DS47273, and DS17690, introduction of penDE gene without introns into these strains in a random manner, via the nonhomologous end-joining pathway. Cooverexpression of penDE with gene phl, encoding the phenylacetic acid CoA ligase leading to production of phenoxyacetyl-CoA and penicillin V | Penicillium chrysogenum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.164 | additional information | in high-yielding penicillin production strains IAT is the limiting factor and that this limitation can be alleviated by a balanced overproduction of this enzyme. Cooverexpression of penDE with gene phl, encoding the phenylacetic acid CoA ligase leading to production of phenoxyacetyl-CoA and penicillin V | Penicillium chrysogenum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.164 | phenylacetyl-CoA + isopenicillin N + H2O | Penicillium chrysogenum | - |
CoA + penicillin G + L-2-aminohexanedioate | - |
? | |
2.3.1.164 | phenylacetyl-CoA + isopenicillin N + H2O | Penicillium chrysogenum DS17690 | - |
CoA + penicillin G + L-2-aminohexanedioate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.164 | Penicillium chrysogenum | - |
gene penDE | - |
2.3.1.164 | Penicillium chrysogenum DS17690 | - |
gene penDE | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.164 | additional information | the protein level of IAT already saturates at low cluster copy numbers, lack of correlation between the protein and transcript levels of the IAT enzyme | Penicillium chrysogenum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.164 | phenoxyacetyl-CoA + isopenicillin N + H2O | - |
Penicillium chrysogenum | CoA + penicillin V + L-2-aminohexanedioate | - |
? | |
2.3.1.164 | phenoxyacetyl-CoA + isopenicillin N + H2O | - |
Penicillium chrysogenum DS17690 | CoA + penicillin V + L-2-aminohexanedioate | - |
? | |
2.3.1.164 | phenylacetyl-CoA + isopenicillin N + H2O | - |
Penicillium chrysogenum | CoA + penicillin G + L-2-aminohexanedioate | - |
? | |
2.3.1.164 | phenylacetyl-CoA + isopenicillin N + H2O | - |
Penicillium chrysogenum DS17690 | CoA + penicillin G + L-2-aminohexanedioate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.164 | IAT | - |
Penicillium chrysogenum |
2.3.1.164 | isopenicillin N acyltransferase | - |
Penicillium chrysogenum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.164 | malfunction | penicillin production in high-yielding strains can be further improved by the overexpression of IAT while at very high levels of IAT the precursor 6-aminopenicillic acid accumulates | Penicillium chrysogenum |
2.3.1.164 | physiological function | IAT catalyzes a limiting step in high-yielding penicillin production strains | Penicillium chrysogenum |