EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.7 | expression in Escherichia coli | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.7 | additional information | - |
additional information | steady-state kinetic parameters for single-base extension reactions by Dpo4 in the presence of either Ca2+ or Mg2+ | Saccharolobus solfataricus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.7 | Ca2+ | Ca2+ (but not Ba2+, Co2+, Cu2+, Ni2+, or Zn2+) is a cofactor for Dpo4-catalyzed polymerization with both native and 8-oxoG-containing DNA templates. Both dNTP and ddNTP are substrates of the polymerase in the presence of either Mg2+ or Ca2+. No pyrophosphorolysis occurs in the presence of Ca2+ | Saccharolobus solfataricus | |
2.7.7.7 | Mg2+ | cofactor for both the polymerase and pyrophosphorolysis activities | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.7 | Saccharolobus solfataricus | Q97W02 | - |
- |
2.7.7.7 | Saccharolobus solfataricus P2 | Q97W02 | - |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.7 | DNA polymerase Dpo4 | - |
Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.7 | additional information | - |
additional information | steady-state kinetic parameters for single-base extension reactions by Dpo4 in the presence of either Ca2+ or Mg2+ | Saccharolobus solfataricus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.7 | additional information | - |
additional information | steady-state kinetic parameters for single-base extension reactions by Dpo4 in the presence of either Ca2+ or Mg2+ | Saccharolobus solfataricus |