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Literature summary extracted from

  • Bauer, R.J.; Begley, M.T.; Trakselis, M.A.
    Kinetics and fidelity of polymerization by DNA polymerase III from Sulfolobus solfataricus (2012), Biochemistry, 51, 1996-2007.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.7 expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.7 D424A mutation in polymerase active site, the polymerase activity is reduced more than 30fold compared to the wild-type activity Saccharolobus solfataricus
2.7.7.7 D424A/D542A mutation in polymerase active site, complete inactivation of polymerase activity Saccharolobus solfataricus
2.7.7.7 D542A mutation in polymerase active site, the polymerase activity is reduced more than 50fold compared to the wild-type activity Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Saccharolobus solfataricus P95979
-
-
2.7.7.7 Saccharolobus solfataricus P2 P95979
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 additional information Dpo3 has an active exonuclease proofreading domain, it shows intrinsic exonuclease activity Saccharolobus solfataricus ?
-
 ?
2.7.7.7 additional information Dpo3 has an active exonuclease proofreading domain, it shows intrinsic exonuclease activity Saccharolobus solfataricus P2 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 DNA polymerase III
-
 Saccharolobus solfataricus
2.7.7.7 Dpo3
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.7 65
-
 polymerization activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.7 70
-
 preincubation at 70°C for 55 min minimally reduces polymerase activity Saccharolobus solfataricus
2.7.7.7 94
-
 estimated melting temperature is avove 94°C, which is increased slightly in the presence of DNA Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.7 additional information
-
 additional information the fidelity of nucleotide incorporation by Dpo3 from the polymerase active site alone is 1000-10000 at 37°C. The functional exonuclease proofreading active site will increase fidelity by at least 100 Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 7 8.5 maximal activity is observed with 20 mM Tris between pH 7 and 8.5 Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
2.7.7.7 physiological function Dpo3 is a potential player in the proper maintenance of the archaeal genome Saccharolobus solfataricus