EC Number | Cloned (Comment) | Organism |
---|---|---|
2.2.1.6 | - |
Pseudomonas sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.2.1.6 | additional information | cloning of a herbicide-resistant acetohydroxyacid synthase gene from Pseudomonas sp. Lm10. Sequence analysis shows that the regulatory subunit of the resisitant enzyme is identical to that of Pseudomonas putida KT2440, whereas six mutations are found in the catalytic subunit, i.e. resistant AHAS/sensitive AHAS: H134N, A135P, S136T, I210V, F264Y, and S486W | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.6 | flumetsulam | herbicide-resistant enzyme variant from Pseudomonas sp. Lm10 shows 6.5fold higher resistance than the sensitive variant from Pseudomonas putida KT2440 | Pseudomonas sp. | |
2.2.1.6 | imazethapyr | herbicide-resistant enzyme variant from Pseudomonas sp. Lm10 shows 12.6fold higher resistance than the sensitive variant from Pseudomonas putida KT2440 | Pseudomonas sp. | |
2.2.1.6 | L-valine | - |
Pseudomonas sp. | |
2.2.1.6 | metsulfuron-methyl | herbicide-resistant enzyme variant from Pseudomonas sp. Lm10 shows 56fold higher resistance than the sensitive variant from Pseudomonas putida KT2440 | Pseudomonas sp. | |
2.2.1.6 | pyriminobac-methyl | herbicide-resistant enzyme variant from Pseudomonas sp. Lm10 shows 9.2fold higher resistance than the sensitive variant from Pseudomonas putida KT2440 | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.6 | 17.5 | - |
pyruvate | pH 7.5, 37°C | Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.6 | Mg2+ | Km value 0.00012 mM | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.2.1.6 | 62000 | - |
x * 62000, SDS-PAGE, catalytic subunit | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.2.1.6 | Pseudomonas sp. | G0ZSE6 | catalytic subunit | - |
2.2.1.6 | Pseudomonas sp. Lm10 | G0ZSE6 | catalytic subunit | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.2.1.6 | 13.5 | - |
pH 7.5, 37°C | Pseudomonas sp. |
EC Number | Storage Stability | Organism |
---|---|---|
2.2.1.6 | -70°C, 3 weeks, loss of 5% of activity | Pseudomonas sp. |
2.2.1.6 | 4°C, 3 weeks, loss of 10% of activity | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.6 | 2 pyruvate | - |
Pseudomonas sp. | 2-acetolactate + CO2 | - |
? | |
2.2.1.6 | 2 pyruvate | - |
Pseudomonas sp. Lm10 | 2-acetolactate + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.2.1.6 | ? | x * 62000, SDS-PAGE, catalytic subunit | Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.6 | FAD | Km value 0.00033 mM | Pseudomonas sp. | |
2.2.1.6 | thiamine diphosphate | Km value 0.0092 mM | Pseudomonas sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.6 | 0.036 | - |
flumetsulam | pH 7.5, 37°C | Pseudomonas sp. | |
2.2.1.6 | 0.065 | - |
pyriminobac-methyl | pH 7.5, 37°C | Pseudomonas sp. | |
2.2.1.6 | 0.105 | - |
L-valine | pH 7.5, 37°C | Pseudomonas sp. | |
2.2.1.6 | 0.121 | - |
imazethapyr | pH 7.5, 37°C | Pseudomonas sp. | |
2.2.1.6 | 0.201 | - |
metsulfuron-methyl | pH 7.5, 37°C | Pseudomonas sp. |