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Literature summary extracted from

  • Seffernick, J.L.; Erickson, J.S.; Cameron, S.; Cho, S.; Dodge, A.G.; Richman, J.; Sadowsky, M.J.; Wackett, L.P.
    Defining the cyanuric acid hydrolase (AtzD)/barbiturase protein family: Sequences and Reactions (2012), J. Bacteriol., 194, 4579-4588.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.15 expression in Escherichia coli Moorella thermoacetica
3.5.2.15 expression in Escherichia coli Acidovorax citrulli
3.5.2.15 expression in Escherichia coli Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.15 0.023
-
 cyanuric acid pH 8.0, 22°C Pseudomonas sp.
3.5.2.15 0.058
-
 cyanuric acid pH 8.0, 22°C Acidovorax citrulli
3.5.2.15 0.11
-
 cyanuric acid pH 8.0, 22°C Moorella thermoacetica

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.15 Acidovorax citrulli
-
-
-
3.5.2.15 Acidovorax citrulli 12227
-
-
-
3.5.2.15 Moorella thermoacetica
-
-
-
3.5.2.15 Pseudomonas sp. P58329
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.15 cyanuric acid + H2O
-
 Moorella thermoacetica carboxybiuret product spontaneously decarboxylates to give biuret and CO2. Therefore cyanuric acid hydrolase and barbiturase catalyze completely analogous reactions ?
3.5.2.15 cyanuric acid + H2O
-
 Acidovorax citrulli carboxybiuret product spontaneously decarboxylates to give biuret and CO2. Therefore cyanuric acid hydrolase and barbiturase catalyze completely analogous reactions ?
3.5.2.15 cyanuric acid + H2O
-
 Pseudomonas sp. carboxybiuret product spontaneously decarboxylates to give biuret and CO2. Therefore cyanuric acid hydrolase and barbiturase catalyze completely analogous reactions ?
3.5.2.15 cyanuric acid + H2O
-
 Acidovorax citrulli 12227 carboxybiuret product spontaneously decarboxylates to give biuret and CO2. Therefore cyanuric acid hydrolase and barbiturase catalyze completely analogous reactions ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.15 AtzD
-
 Pseudomonas sp.
3.5.2.15 TrzD
-
 Acidovorax citrulli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.15 10.6
-
 cyanuric acid pH 8.0, 22°C Moorella thermoacetica
3.5.2.15 14.2
-
 cyanuric acid pH 8.0, 22°C Acidovorax citrulli
3.5.2.15 73
-
 cyanuric acid pH 8.0, 22°C Pseudomonas sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.2.15 100
-
 cyanuric acid pH 8.0, 22°C Moorella thermoacetica
3.5.2.15 2500
-
 cyanuric acid pH 8.0, 22°C Acidovorax citrulli
3.5.2.15 3200
-
 cyanuric acid pH 8.0, 22°C Pseudomonas sp.