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Literature summary extracted from

  • Verdon, G.; Albers, S.V.; van Oosterwijk, N.; Dijkstra, B.W.; Driessen, A.J.; Thunnissen, A.M.
    Formation of the productive ATP-Mg2+-bound dimer of GlcV, an ABC-ATPase from Sulfolobus solfataricus (2003), J. Mol. Biol., 334, 255-267.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
7.5.2.B8 Crystals of the G144A mutant are grown by microseeding from wild-type GlcV crystals in hanging-drop vapour-diffusion Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
7.5.2.B8 E166A mutants shows no significant activity, and shows dimerization in the presence of ATP-Mg2+ Saccharolobus solfataricus
7.5.2.B8 E166Q mutant lost about 80% of the wild-type activity, it shows dimerization in the presence of ATP-Mg2+ or ATP alone Saccharolobus solfataricus
7.5.2.B8 G144A mutant is completely inactive and fails to dimerize, indicating an essential role of this residue in stabilizing the productive dimeric state. Although the E166A and G144A mutants each alone are inactive, they produce an active heterodimer, showing that disruption of one active site can be tolerated Saccharolobus solfataricus
7.5.2.B8 S142A mutant retains considerable activity, and is able to dimerize, thus implying that the interaction of the serine with ATP is not essential for dimerization and catalysis. Although the E166A and G144A mutants each alone are inactive, they produce an active heterodimer, showing that disruption of one active site can be tolerated Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.5.2.B8 40000
-
 in absence of nucleotide-Mg2+, monomer, gel filtration Saccharolobus solfataricus
7.5.2.B8 60000
-
 dimer, this molecular mass of 60 kDa can be explained by the occurrence of a fast equilibrium between monomeric and dimeric states, gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
7.5.2.B8 Saccharolobus solfataricus Q97UY8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.5.2.B8
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.5.2.B8 ATP + H2O + D-glucose/out
-
 Saccharolobus solfataricus ADP + phosphate + D-glucose/in
-
 ?

Subunits

EC Number Subunits Comment Organism
7.5.2.B8 dimer binding of ATP and Mg2+ to monomeric GlcV results in the formation of a productive dimer Saccharolobus solfataricus
7.5.2.B8 monomer isolated enzyme behaves apparently as a monomer in the presence of ATP-Mg2+, AMPPNP-Mg2+ or ATP alone Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
7.5.2.B8 ABC transporter, ATP binding protein (glucose)
-
 Saccharolobus solfataricus
7.5.2.B8 ABC-ATPase GlcV
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.5.2.B8 70
-
 assay at Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5.2.B8 6.5
-
 assay at Saccharolobus solfataricus