Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Boudsocq, F.; Iwai, S.; Hanaoka, F.; Woodgate, R.
    Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic poleta (2001), Nucleic Acids Res., 29, 4607-4616.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.7
-
 Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 40189
-
 x * 40189, calculated from sequence Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Saccharolobus solfataricus P96022
-
-
2.7.7.7 Saccharolobus solfataricus P2 P96022
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn in the absence of additional cofactor the enzyme is an essentially distributive enzyme that only extends primers by 1-2 nt per binding event. At high enzyme to primer/template ratios, dissociation and rebinding of the enzyme to the primer/template is robust and can lead to the synthesis of polynucleotide chains of several hundred nucleotides in length Saccharolobus solfataricus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn in the absence of additional cofactor the enzyme is an essentially distributive enzyme that only extends primers by 1-2 nt per binding event. At high enzyme to primer/template ratios, dissociation and rebinding of the enzyme to the primer/template is robust and can lead to the synthesis of polynucleotide chains of several hundred nucleotides in length Saccharolobus solfataricus P2 diphosphate + DNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.7 ? x * 40189, calculated from sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 DNA polymerase IV
-
 Saccharolobus solfataricus
2.7.7.7 Dpo4
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.7 37 95 high activity in the temperature range Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.7 85
-
 5 min, enzyme is not denatured after heating of crude cell lysate Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.7 Saccharolobus solfataricus calculated from sequence
-
 9.1