Literature summary extracted from
DAmbrosio, K.; Limauro, D.; Pedone, E.; Galdi, I.; Pedone, C.; Bartolucci, S.; De Simone, G.
Insights into the catalytic mechanism of the Bcp family: Functional and structural analysis of Bcp1 from Sulfolobus solfataricus (2009), Proteins Struct. Funct. Bioinform., 76, 995-1006.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.11.1.24 |
mutant enzymes are overproduced in soluble form as fusion with a C-terminal eight-residue histidine tag in Escherichia coli BL21 |
Saccharolobus solfataricus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.11.1.24 |
hanging-drop vapor-diffusion method, crystal structure at 2.15 A, resolution of the C45S/C50S double mutant |
Saccharolobus solfataricus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.11.1.24 |
C45S |
mutation in cysteine 45 results in complete loss of DTT-dependent peroxidase activity |
Saccharolobus solfataricus |
1.11.1.24 |
C50S |
the C50S mutant enzyme retains almost all the activity of the wild-type enzyme (DTT-dependent peroxidase activity). Unlike the wild-type enzyme, the mutant enzyme is able to form in vitro a homodimer via an intermolecular disulfide bond |
Saccharolobus solfataricus |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.11.1.24 |
18525 |
- |
x * 18525, calculated from sequence |
Saccharolobus solfataricus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.11.1.24 |
Saccharolobus solfataricus |
Q97WP9 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.11.1.24 |
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.11.1.24 |
H2O2 + dithiothreitol |
- |
Saccharolobus solfataricus |
H2O + oxidized dithiothreitol |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.11.1.24 |
? |
x * 18525, calculated from sequence |
Saccharolobus solfataricus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.11.1.24 |
DTT-dependent peroxidase |
- |
Saccharolobus solfataricus |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.11.1.24 |
80 |
- |
assay at |
Saccharolobus solfataricus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.11.1.24 |
7 |
- |
assay at |
Saccharolobus solfataricus |