EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.42 | gene Rv3339c, expression of N-terminally His6-tagged enzyme | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.42 | purified ICDH-1 in complex with NADPH at Mn2+, hanging drop vapor diffusion method, 0.001 ml of protein in 5 mM NADPH, and 5 mM MnCl2 is mixed with 0.001 ml of reservoir solution containing 30% PEG 2000 and 0.1 M Tris-HCl, pH 8.0, room temperature, X-ray diffraction structure determination and analysis at 2.18 A resoltuion | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.42 | 2-oxoglutarate | noncompetitive versus NADP+ | Mycobacterium tuberculosis | |
1.1.1.42 | malate | noncompetitive versus NADP+ and isocitrate | Mycobacterium tuberculosis | |
1.1.1.42 | additional information | product inhibition patterns for ICDH-1, overview | Mycobacterium tuberculosis | |
1.1.1.42 | NADPH | product inhibition competitive versus NADP+ and noncompetitive versus isocitrate | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.42 | additional information | - |
additional information | steady-state kinetics, kinetic mechanism of ICDH-1, overview | Mycobacterium tuberculosis | |
1.1.1.42 | 0.015 | - |
NADP+ | pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme | Mycobacterium tuberculosis | |
1.1.1.42 | 0.05 | - |
isocitrate | pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.42 | Mn2+ | required | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.42 | isocitrate + NADP+ | Mycobacterium tuberculosis | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | Mycobacterium tuberculosis H37Rv | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.42 | Mycobacterium tuberculosis | P9WKL1 | gene Rv3339c | - |
1.1.1.42 | Mycobacterium tuberculosis H37Rv | P9WKL1 | gene Rv3339c | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.42 | recombinant N-terminally His6-tagged enzyme by nickel affinity chromatography and gel filtration | Mycobacterium tuberculosis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.42 | isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH + H+ | protonation of the enolate to form product 2-oxoglutarate is the rate-limiting step | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.42 | isocitrate + NADP+ | - |
Mycobacterium tuberculosis | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | - |
Mycobacterium tuberculosis H37Rv | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | additional information | Mycobacterium tuberculosis ICDH-1 also catalyzes the formation of 2-hydroxyglutarate | Mycobacterium tuberculosis | ? | - |
? | |
1.1.1.42 | additional information | Mycobacterium tuberculosis ICDH-1 also catalyzes the formation of 2-hydroxyglutarate | Mycobacterium tuberculosis H37Rv | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.42 | homodimer | each subunit has a Rossmann fold, and a common top domain of interlocking beta sheets | Mycobacterium tuberculosis |
1.1.1.42 | More | three-dimensional structure of Mtb ICDH-1, overview | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.42 | ICDH | - |
Mycobacterium tuberculosis |
1.1.1.42 | ICDH-1 | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.42 | 33 | - |
NADP+ | pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme | Mycobacterium tuberculosis | |
1.1.1.42 | 33 | - |
isocitrate | pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.42 | 7.5 | - |
assay at | Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.42 | NADP+ | - |
Mycobacterium tuberculosis |