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Literature summary extracted from

  • Tachibana, Y.; Mendez Leclerre, M.; Fujiwara, S.; Takagi, M.; Imanaka, T.
    Cloning and expression of the alpha-amylase gene from the hyperthermophilic archaeon Pyrococcus sp. KOD 1, and characterization of the enzyme (1996), J. Ferment. Bioeng., 82, 224-232.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expression in Escherichia coli, T7 RNA polymerase expression system Pyrococcus sp.

General Stability

EC Number General Stability Organism
3.2.1.1 no loss of activity after dialysis of the purified enzyme overnight at 4°C against 100 mM MES buffer (pH 6.5) containing 1 mM EDTA Pyrococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 alpha-cyclodextrin 0.2%, 10% inhibition Pyrococcus sp.
3.2.1.1 beta-cyclodextrin 0.2%, 19% inhibition Pyrococcus sp.
3.2.1.1 CoCl2 1 mM, 52% inhibition Pyrococcus sp.
3.2.1.1 CuCl2 1 mM, complete inhibition Pyrococcus sp.
3.2.1.1 gamma-cyclodextrin 0.2%, 18% inhibition Pyrococcus sp.
3.2.1.1 glucosyl-alpha-cyclodextrin 0.2%, 19% inhibition Pyrococcus sp.
3.2.1.1 MnCl2 1 mM, 55% inhibition Pyrococcus sp.
3.2.1.1 SDS 1.0% 63% inhibition Pyrococcus sp.
3.2.1.1 ZnCl2 1 mM, complete inhibition Pyrococcus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.1 extracellular
-
 Pyrococcus sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 42000
-
 x * 42000, SDS-PAGE Pyrococcus sp.
3.2.1.1 49456
-
 x * 49456, calculated from sequence Pyrococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Pyrococcus sp. O33476
-
-
3.2.1.1 Pyrococcus sp. KOD1 O33476
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1
-
 Pyrococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 amylopectin + H2O
-
 Pyrococcus sp. ?
-
 ?
3.2.1.1 amylopectin + H2O
-
 Pyrococcus sp. KOD1 ?
-
 ?
3.2.1.1 amylose + H2O
-
 Pyrococcus sp. ?
-
 ?
3.2.1.1 amylose + H2O
-
 Pyrococcus sp. KOD1 ?
-
 ?
3.2.1.1 glycogen + H2O
-
 Pyrococcus sp. maltose + maltotriose major products ?
3.2.1.1 glycogen + H2O
-
 Pyrococcus sp. KOD1 maltose + maltotriose major products ?
3.2.1.1 additional information very weakly hydrolyzes pullulan to form panose or isopanose Pyrococcus sp. ?
-
 ?
3.2.1.1 additional information very weakly hydrolyzes pullulan to form panose or isopanose Pyrococcus sp. KOD1 ?
-
 ?
3.2.1.1 starch + H2O
-
 Pyrococcus sp. maltose + maltotriose major products ?
3.2.1.1 starch + H2O
-
 Pyrococcus sp. KOD1 maltose + maltotriose major products ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 42000, SDS-PAGE Pyrococcus sp.
3.2.1.1 ? x * 49456, calculated from sequence Pyrococcus sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 ApkA
-
 Pyrococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 90
-
-
 Pyrococcus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 60 100 60°C: about 60% of maximal activity, 100°C: about 80% of maximal activity Pyrococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 90
-
 60 min, about 10% loss of activity, thermostability is enhanced in the presence of 2 mM Ca2+ Pyrococcus sp.
3.2.1.1 100
-
 60 min, in presence of 2 mM Ca2+, about 60% loss of activity Pyrococcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.1 2280
-
 amylose pH 6.5, 90°C Pyrococcus sp.
3.2.1.1 2300
-
 glycogen pH 6.5, 90°C Pyrococcus sp.
3.2.1.1 4180
-
 amylopectin pH 6.5, 90°C Pyrococcus sp.
3.2.1.1 4680
-
 starch pH 6.5, 90°C Pyrococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 6.5
-
-
 Pyrococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 4.5 8 pH 4.5: about 40% of maximal activity, pH 8.0: about 40% of maximal activity Pyrococcus sp.