Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Menckhoff, L.; Mielke-Ehret, N.; Buck, F.; Vuletic, M.; Luethje, S.
    Plasma membrane-associated malate dehydrogenase of maize (Zea mays L.) roots: native versus recombinant protein (2013), J. Proteomics, 80C, 66-77.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.37 from roots, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.025
-
 NADH pH 7.5, temperature not specified in the publication, native enzyme Zea mays
1.1.1.37 0.029
-
 NADH pH 7.5, temperature not specified in the publication, recombinant enzyme Zea mays
1.1.1.37 0.126
-
 oxaloacetate pH 7.5, temperature not specified in the publication, native enzyme Zea mays
1.1.1.37 0.289
-
 oxaloacetate pH 7.5, temperature not specified in the publication, recombinant enzyme Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.37 cytosol
-
 Zea mays 5829
-
1.1.1.37 plasma membrane associated Spinacia oleracea 5886
-
1.1.1.37 plasma membrane associated Zea mays 5886
-
1.1.1.37 plasma membrane associated Brassica oleracea 5886
-
1.1.1.37 soluble
-
 Zea mays
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 35000
-
 x * 35000, recombinant enzyme, SDS-PAGE, x * 35560, plasma membrane-associated enzyme, sequence calculation Zea mays
1.1.1.37 35560
-
 x * 35000, recombinant enzyme, SDS-PAGE, x * 35560, plasma membrane-associated enzyme, sequence calculation Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 (S)-malate + NAD+ Spinacia oleracea
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+ Zea mays
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+ Brassica oleracea
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+ Zea mays Goldener Badischer Landmais
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 oxaloacetate + NADH + H+ Zea mays
-
 (S)-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH + H+ Zea mays Goldener Badischer Landmais
-
 (S)-malate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Brassica oleracea
-
 two isozymes
-
1.1.1.37 Spinacia oleracea
-
-
-
1.1.1.37 Zea mays
-
-
-
1.1.1.37 Zea mays Goldener Badischer Landmais
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37 native enzyme from leaves bound to plasma membranes by affinity chromatography Spinacia oleracea
1.1.1.37 native enzyme from leaves, and two isozymes from roots bound to plasma membranes by affinity chromatography, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Zea mays
1.1.1.37 native two isozymes from inflorescences bound to plasma membranes by affinity chromatography Brassica oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 inflorescence
-
 Brassica oleracea
-
1.1.1.37 leaf
-
 Spinacia oleracea
-
1.1.1.37 leaf PM-associated MDH and soluble MDH isozymes Zea mays
-
1.1.1.37 root root isozymes Zea mays
-
1.1.1.37 seed PM-associated MDH isozyme Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Spinacia oleracea oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Zea mays oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Brassica oleracea oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Zea mays Goldener Badischer Landmais oxaloacetate + NADH + H+
-
 r
1.1.1.37 oxaloacetate + NADH + H+
-
 Zea mays (S)-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH + H+
-
 Zea mays Goldener Badischer Landmais (S)-malate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.37 ? x * 35000, recombinant enzyme, SDS-PAGE, x * 35560, plasma membrane-associated enzyme, sequence calculation Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 7.4
-
 recombinant enzyme Zea mays
1.1.1.37 7.5
-
 assay at Spinacia oleracea
1.1.1.37 7.5
-
 assay at Brassica oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.37 NAD+
-
 Spinacia oleracea
1.1.1.37 NAD+
-
 Zea mays
1.1.1.37 NAD+
-
 Brassica oleracea
1.1.1.37 NADH
-
 Spinacia oleracea
1.1.1.37 NADH
-
 Zea mays
1.1.1.37 NADH
-
 Brassica oleracea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.37 Zea mays plasma membrane-associated enzyme, sequence calculation
-
 5.75
1.1.1.37 Zea mays purified recombinant enzyme, isoelectric focussing
-
 6.8

General Information

EC Number General Information Comment Organism
1.1.1.37 evolution the plasma membrane-associated isozyme belongs belongs to the lactate dehydrogenase/MDH superfamily, MDH type 2 family Zea mays
1.1.1.37 physiological function besides its function in malate synthesis, MDH is responsible for the exchange of reducing equivalents between metabolic pathways in distinct cell compartments Spinacia oleracea
1.1.1.37 physiological function besides its function in malate synthesis, MDH is responsible for the exchange of reducing equivalents between metabolic pathways in distinct cell compartments Zea mays
1.1.1.37 physiological function besides its function in malate synthesis, MDH is responsible for the exchange of reducing equivalents between metabolic pathways in distinct cell compartments Brassica oleracea