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Literature summary extracted from

  • Decker, D.; Meng, M.; Gornicka, A.; Hofer, A.; Wilczynska, M.; Kleczkowski, L.A.
    Substrate kinetics and substrate effects on the quaternary structure of barley UDP-glucose pyrophosphorylase (2012), Phytochemistry, 79, 39-45.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.9 expression in Escherichia coli Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.9 additional information
-
 additional information kinetics, overview Hordeum vulgare
2.7.7.9 0.025
-
 UTP pH 7.5, temperature not specified in the publication Hordeum vulgare
2.7.7.9 0.033
-
 alpha-D-glucose 1-phosphate pH 7.5, temperature not specified in the publication Hordeum vulgare
2.7.7.9 10
-
 alpha-D-galactose 1-phosphate pH 7.5, temperature not specified in the publication Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.9 UTP + alpha-D-glucose 1-phosphate Hordeum vulgare
-
 diphosphate + UDP-glucose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Hordeum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.9 recombinant enzyme from Escherichia coli Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.9 leaf
-
 Hordeum vulgare
-
2.7.7.9 root
-
 Hordeum vulgare
-
2.7.7.9 seed developing seeds Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 additional information the enzyme catalyses a freely reversible reaction and is specific for alpha-D-glucose 1-phosphate Hordeum vulgare ?
-
 ?
2.7.7.9 UTP + alpha-D-galactose 1-phosphate low activity Hordeum vulgare diphosphate + UDP-galactose
-
 r
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Hordeum vulgare diphosphate + UDP-glucose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.9 dimer inactive enzyme form Hordeum vulgare
2.7.7.9 monomer active enzyme form Hordeum vulgare
2.7.7.9 More structure modeling, overview. The quaternary structure of the enzyme is affected by addition of either single or both substrates in either direction of the reaction, resulting in a shift from UGPase dimers toward monomers, the active form of the enzyme. The substrate-induced changes in quaternary structure of the enzyme may have a regulatory role to assure maximal activity. The ratio of monomers to dimers is about 5:1 in absence of substrate Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
2.7.7.9 UDP-Glc pyrophosphorylase
-
 Hordeum vulgare
2.7.7.9 UDP-glucose pyrophosphorylase
-
 Hordeum vulgare
2.7.7.9 UGPase
-
 Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.9 6.5 8.5 broad optimum in both reaction directions Hordeum vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.9 4.5 10 forward reaction sharp drop in activity above pH 10.0, inactive above pH 10.0 and below pH 4.0, profile overview Hordeum vulgare
2.7.7.9 5.5 9.5 reverse reaction, inactive above pH 10.0 and below pH 5.0, profile overview Hordeum vulgare

General Information

EC Number General Information Comment Organism
2.7.7.9 evolution the plant UGPases belongs to the so called UGPase-A family Hordeum vulgare
2.7.7.9 additional information structure modeling, overview. The quaternary structure of the enzyme is affected by addition of either single or both substrates in either direction of the reaction, resulting in a shift from UGPase dimers toward monomers, the active form of the enzyme. The substrate-induced changes in quaternary structure of the enzyme may have a regulatory role to assure maximal activity Hordeum vulgare
2.7.7.9 physiological function UDP-Glc pyrophosphorylase is an essential enzyme responsible for production of UDP-Glc, which is used in hundreds of glycosylation reactions involving addition of Glc to a variety of compounds Hordeum vulgare