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Literature summary extracted from

  • Widdel, F.; Wolfe, R.S.
    Expression of secondary alcohol dehydrogenase in methanogenic bacteria and purification of the F420-specific enzyme from Methanogenium thermophilum strain TCI (1989), Arch. Microbiol., 152, 322-328.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.98.5 K2SO4 100 mM, more than 90% inhibition Methanoculleus thermophilus
1.1.98.5 KCl 100 mM, more than 90% inhibition Methanoculleus thermophilus
1.1.98.5 Na2SO4 100 mM, more than 90% inhibition Methanoculleus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.98.5 0.25
-
 propanone pH 4.2, 40°C Methanoculleus thermophilus
1.1.98.5 2.5
-
 2-propanol pH 4.2, 40°C Methanoculleus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.98.5 39500
-
 2 * 39500, SDS-PAGE Methanoculleus thermophilus
1.1.98.5 68000
-
 gel filtration Methanoculleus thermophilus
1.1.98.5 100000
-
 ultrafiltration Methanoculleus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.1.98.5 Methanoculleus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.98.5
-
 Methanoculleus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.98.5 110
-
 2-propanone, pH 4.2, 40°C Methanoculleus thermophilus
1.1.98.5 176
-
 2-propanol, pH 4.2, 40°C Methanoculleus thermophilus

Storage Stability

EC Number Storage Stability Organism
1.1.98.5 4°C, almost no loss in specific activity after 1 year Methanoculleus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.98.5 2-propanol + oxidized coenzyme F420
-
 Methanoculleus thermophilus propanone + reduced coenzyme F420 propanone i.e. acetone r
1.1.98.5 additional information activity with ethanol or 1-propanol at the same concentration is only 0.1% compared to activity with 2-propanol. Acetaldehyde and propionaldehyde are reduced at the same rate as 2-propanone Methanoculleus thermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.98.5 homodimer 2 * 39500, SDS-PAGE Methanoculleus thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.1.98.5 F420-specific sec-ADH
-
 Methanoculleus thermophilus
1.1.98.5 F420-specific secondary alcohol dehydrogenase
-
 Methanoculleus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.98.5 55
-
 pH 5.8, the activity of a 20-fold dilution decreases to 5% within 20 min. If 100 mM potassium sulfate is present 40% of the activity is left after 20 min Methanoculleus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.98.5 4.2
-
-
 Methanoculleus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.98.5 3 7 pH 3.0: about 30% of maximal activity, pH 7.0: about 30% of maximal activity Methanoculleus thermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.98.5 5.8
-
 activity loss of a 50fold diluted extract tested at 0-28°C is least around pH 5.8 (30% in 23 days at 23°C) Methanoculleus thermophilus

Expression

EC Number Organism Comment Expression
1.1.98.5 Methanoculleus thermophilus induced by its substrate, expression is faster if H2 and CO2 are present as energy source up