EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.4 | expression in Escherichia coli | Saccharolobus solfataricus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.4 | Y41C | no structural modifications in the Y41C mutant, the pH-spectrum is slightly increased | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.4 | 0.1 | - |
4-hydroxybenzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 0.1 | - |
2-toluamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 0.1 | - |
4-hydroxybenzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 0.2 | - |
cyclomaltohexaose | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 0.2 | - |
4-nitrobenzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 0.2 | - |
2-toluamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 0.3 | - |
metacrylamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 0.3 | - |
cyclomaltohexaose | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 0.3 | - |
4-nitrobenzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 0.4 | - |
nicotinamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 0.5 | - |
metacrylamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 0.6 | - |
Benzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 0.8 | - |
indol-3-acetamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 0.8 | - |
indol-3-acetamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 0.9 | - |
Benzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 1.5 | - |
nicotinamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 1.5 | - |
Pentanamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 2.5 | - |
Pentanamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 2.5 | - |
3-phenylpropionamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 3 | - |
3-phenylpropionamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 3.2 | - |
butyramide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 4.5 | - |
hexanamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 4.5 | - |
hexanamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 6.5 | - |
Isobutyramide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 7 | - |
Isobutyramide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 10 | - |
Propionamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 10 | - |
Propionamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 14 | - |
butyramide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 22 | - |
acetamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 37 | - |
acetamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.4 | 55784 | - |
x * 55784, calculated from amino acid sequence | Saccharolobus solfataricus |
3.5.1.4 | 83000 | - |
dimeric enzyme form, gel filtration | Saccharolobus solfataricus |
3.5.1.4 | 400000 | - |
octameric enzyme form, gel filtration | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.4 | Saccharolobus solfataricus | P95896 | - |
- |
3.5.1.4 | Saccharolobus solfataricus P2 | P95896 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.4 | - |
Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.4 | 2-toluamide + H2O | - |
Saccharolobus solfataricus | 2-toluic acid + NH3 | - |
? | |
3.5.1.4 | 3-phenylpropionamide + H2O | - |
Saccharolobus solfataricus | 3-phenylpropionate + NH3 | - |
? | |
3.5.1.4 | 4-hydroxybenzamide + H2O | - |
Saccharolobus solfataricus | 4-hydroxybenzoate + NH3 | - |
? | |
3.5.1.4 | 4-methylbenzamide + H2O | - |
Saccharolobus solfataricus | 4-methylbenzoic acid + NH3 | - |
? | |
3.5.1.4 | 4-nitrobenzamide + H2O | - |
Saccharolobus solfataricus | 4-nitrobenzoate + NH3 | - |
? | |
3.5.1.4 | 4-toluamide + H2O | - |
Saccharolobus solfataricus | 4-toluic acid + NH3 | - |
? | |
3.5.1.4 | acetamide + H2O | - |
Saccharolobus solfataricus | acetic acid + NH3 | - |
? | |
3.5.1.4 | acetamide + H2O | - |
Saccharolobus solfataricus P2 | acetic acid + NH3 | - |
? | |
3.5.1.4 | benzamide + H2O | - |
Saccharolobus solfataricus | benzoate + NH3 | - |
? | |
3.5.1.4 | butyramide + H2O | - |
Saccharolobus solfataricus | butyrate + NH3 | - |
? | |
3.5.1.4 | butyramide + H2O | - |
Saccharolobus solfataricus P2 | butyrate + NH3 | - |
? | |
3.5.1.4 | hexanamide + H2O | - |
Saccharolobus solfataricus | hexanoate + NH3 | - |
? | |
3.5.1.4 | indole-3-acetamide + H2O | - |
Saccharolobus solfataricus | indole-3-acetate + NH3 | - |
? | |
3.5.1.4 | isobutyramide + H2O | - |
Saccharolobus solfataricus | isobutyrate + NH3 | - |
? | |
3.5.1.4 | isobutyramide + H2O | - |
Saccharolobus solfataricus P2 | isobutyrate + NH3 | - |
? | |
3.5.1.4 | methacrylamide + H2O | - |
Saccharolobus solfataricus | methacrylate + NH3 | - |
? | |
3.5.1.4 | nicotinamide + H2O | - |
Saccharolobus solfataricus | nicotinic acid + NH3 | - |
? | |
3.5.1.4 | pentanamide + H2O | - |
Saccharolobus solfataricus | pentanoate + NH3 | - |
? | |
3.5.1.4 | pentanamide + H2O | - |
Saccharolobus solfataricus P2 | pentanoate + NH3 | - |
? | |
3.5.1.4 | propionamide + H2O | - |
Saccharolobus solfataricus | propionic acid + NH3 | - |
? | |
3.5.1.4 | propionamide + H2O | - |
Saccharolobus solfataricus P2 | propionic acid + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.4 | ? | x * 55784, calculated from amino acid sequence | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.4 | signature amidase | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.4 | 70 | - |
assay at | Saccharolobus solfataricus |
3.5.1.4 | 95 | - |
- |
Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.4 | additional information | - |
thermal unfolding is not reversible | Saccharolobus solfataricus |
3.5.1.4 | 70 | - |
thermal stability of the wild-type enzyme and its mutant Y41C at pH 5.0 and 70 °C, are similar, with half-life values over 70 h | Saccharolobus solfataricus |
3.5.1.4 | 80 | - |
half-life of wild-type enzyme: 65 h at pH 5.0, 25 h at pH 8.0. half-life of mutant enzyme Y41C: 64 h at pH 5.0, 0.5 h at pH 8.0 | Saccharolobus solfataricus |
3.5.1.4 | 84 | - |
Tm-value of mutant enzyme Y41C at pH 8.0 is 83.5°C | Saccharolobus solfataricus |
3.5.1.4 | 90 | - |
half-life of wild-type enzyme: 60 h at pH 5.0, 0.5 h at pH 8.0. half-life of mutant enzyme Y41C: 20 h at pH 5.0, 0.25 h at pH 8.0 | Saccharolobus solfataricus |
3.5.1.4 | 94 | - |
Tm-value of wild-type enzyme at pH 8.0 is 94.4°C | Saccharolobus solfataricus |
3.5.1.4 | 97 | - |
Tm-value of mutant enzyme Y41C at pH 5.0 is 97.3°C | Saccharolobus solfataricus |
3.5.1.4 | 104 | - |
Tm-value of wild-type enzyme at pH 5.0 is 104.3°C | Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.4 | 0.4 | - |
2-toluamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 0.8 | - |
4-nitrobenzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 0.8 | - |
2-toluamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 1.2 | - |
indol-3-acetamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 1.9 | - |
4-hydroxybenzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 2.1 | - |
4-hydroxybenzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 2.2 | - |
indol-3-acetamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 2.2 | - |
nicotinamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 2.5 | - |
4-nitrobenzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 3 | - |
metacrylamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 4.8 | - |
metacrylamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 6.4 | - |
nicotinamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 6.7 | - |
Benzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 7.2 | - |
Benzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 12.2 | - |
4-toluamide | wild type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 12.2 | - |
4-toluamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 14.2 | - |
4-toluamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 14.2 | - |
4-toluamide | mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 15.3 | - |
3-phenylpropionamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 17.5 | - |
butyramide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 18 | - |
3-phenylpropionamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 19.5 | - |
hexanamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 21.6 | - |
hexanamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 26.8 | - |
Pentanamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 28.6 | - |
Pentanamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 28.9 | - |
acetamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 30.1 | - |
acetamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 34.3 | - |
butyramide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 48.9 | - |
Isobutyramide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 58.4 | - |
Isobutyramide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 82.9 | - |
Propionamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 99.4 | - |
Propionamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.4 | 5 | - |
at 70°C, assay at | Saccharolobus solfataricus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.4 | 3.5 | 8.5 | pH 3.5: wild-type enzyme shows about 45% of maximal activity, mutant enzyme Y41C shows about 20% of maximal activity. pH 8.5: wild-type enzyme shows about 40% of maximal activity, mutant enzyme Y41C shows about 50% of maximal activity | Saccharolobus solfataricus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.4 | 0.81 | - |
acetamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 1.31 | - |
acetamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 1.5 | - |
indol-3-acetamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 2.45 | - |
butyramide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 2.75 | - |
indol-3-acetamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 4 | - |
2-toluamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 4 | - |
4-nitrobenzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 4 | - |
2-toluamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 4.26 | - |
nicotinamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 4.33 | - |
hexanamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 4.8 | - |
hexanamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 5.1 | - |
3-phenylpropionamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 5.47 | - |
butyramide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 5.5 | - |
nicotinamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 6 | - |
metacrylamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 6.99 | - |
Isobutyramide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 7.2 | - |
3-phenylpropionamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 7.44 | - |
Benzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 8.29 | - |
Propionamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 8.33 | - |
4-nitrobenzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 8.98 | - |
Isobutyramide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 9.94 | - |
Propionamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 10.7 | - |
Pentanamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 12 | - |
Benzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 16 | - |
metacrylamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 19 | - |
4-hydroxybenzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.5.1.4 | 19 | - |
Pentanamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 21 | - |
4-hydroxybenzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 47.3 | - |
cyclomaltohexaose | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3.5.1.4 | 61 | - |
cyclomaltohexaose | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus |