EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.284 | real-time PCR expression analysis, recombinant expression in Escherichia coli as N-terminally His-tagged enzyme | Solanum lycopersicum |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.284 | purified recombinant His-tagged enzyme, in complex with NAD+ and with NADH, hanging drop vapour diffusion, mixing of 0.003 ml of 22 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 2.5 mM NAD+, or 2.5 mM NADH and 5.0 mM GSH, 20°C, X-ray diffraction tructure determination and analysis at 1.85-2.14 A resolution, molecular replacement and modeling | Solanum lycopersicum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.284 | 2-mercaptoethanol | inhibits both the reductase and dehydrogenase reactions by 30% at 1 mM | Solanum lycopersicum | |
1.1.1.284 | Ag+ | - |
Solanum lycopersicum | |
1.1.1.284 | ascorbic acid | inhibits both the reductase and dehydrogenase reactions by 30% at 1 mM | Solanum lycopersicum | |
1.1.1.284 | Decanoic acid | - |
Solanum lycopersicum | |
1.1.1.284 | dithiothreitol | inhibits both the reductase and dehydrogenase reactions by 30% at 1 mM | Solanum lycopersicum | |
1.1.1.284 | dodecanoic acid | noncompetitive | Solanum lycopersicum | |
1.1.1.284 | EDTA | complete inactivation | Solanum lycopersicum | |
1.1.1.284 | glutathione | noncompetitive inhibitor, inhibits GSNO reduction | Solanum lycopersicum | |
1.1.1.284 | Hg2+ | - |
Solanum lycopersicum | |
1.1.1.284 | N6022 | noncompetitive | Solanum lycopersicum | |
1.1.1.284 | octanoic acid | - |
Solanum lycopersicum | |
1.1.1.284 | S-Methylglutathione | noncompetitive inhibitor, inhibits GSNO reduction | Solanum lycopersicum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.284 | 0.04 | - |
NAD+ | pH 8.0, 30°C, recombinant enzyme | Solanum lycopersicum | |
1.1.1.284 | 0.057 | - |
S-nitrosylglutathione | pH 8.0, 30°C, recombinant enzyme | Solanum lycopersicum | |
1.1.1.284 | 0.058 | - |
NADH | pH 8.0, 30°C, recombinant enzyme | Solanum lycopersicum | |
1.1.1.284 | 0.058 | - |
S-(hydroxymethyl)glutathione | pH 8.0, 30°C, recombinant enzyme | Solanum lycopersicum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.284 | cytosol | - |
Solanum lycopersicum | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.284 | additional information | other monovalent and bivalent cations, besides Hg2+ or Ag+, have only a small effect | Solanum lycopersicum | |
1.1.1.284 | Zn2+ | required for the oxidation reaction. The catalytic zinc ion functions as a Lewis acid, is bound by Cys177 and Cys47, His69 and either Glu70 or water (hydroxide anion). The structural zinc is bound by four cysteines, Cys99, Cys102, Cys105, and Cys113 | Solanum lycopersicum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.284 | 40000 | - |
2 * 40000, SDS-PAGE | Solanum lycopersicum |
1.1.1.284 | 81090 | - |
native enzyme, sequence calculation | Solanum lycopersicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.284 | S-(hydroxymethyl)glutathione + NAD+ | Solanum lycopersicum | - |
S-formylglutathione + NADH + H+ | - |
? | |
1.1.1.284 | S-(hydroxymethyl)glutathione + NAD+ | Solanum lycopersicum Amateur | - |
S-formylglutathione + NADH + H+ | - |
? | |
1.1.1.284 | S-nitrosoglutathione + NADH | Solanum lycopersicum | - |
? + NAD+ | a variety of products depending on cellular conditions, including glutathione disulfide, glutathione sulfinamide and hydroxylamine | ? | |
1.1.1.284 | S-nitrosoglutathione + NADH | Solanum lycopersicum Amateur | - |
? + NAD+ | a variety of products depending on cellular conditions, including glutathione disulfide, glutathione sulfinamide and hydroxylamine | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.284 | Solanum lycopersicum | D2Y3F4 | - |
- |
1.1.1.284 | Solanum lycopersicum Amateur | D2Y3F4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.284 | recombinant N-terminally His-tagged enzyme from Escherichia coli by methal chelating chromatography | Solanum lycopersicum |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.284 | S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H + H+ | structure-function analysis, active site and ligand binding structures,reaction mechanism, overview | Solanum lycopersicum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.284 | cotyledon | - |
Solanum lycopersicum | - |
1.1.1.284 | flower | - |
Solanum lycopersicum | - |
1.1.1.284 | fruit | the enzyme is highly expressed in the pistil and stamens and in fruits during ripening | Solanum lycopersicum | - |
1.1.1.284 | leaf | - |
Solanum lycopersicum | - |
1.1.1.284 | additional information | expression is higher in roots and stems compared to leaves of young plants | Solanum lycopersicum | - |
1.1.1.284 | pistil | - |
Solanum lycopersicum | - |
1.1.1.284 | root | - |
Solanum lycopersicum | - |
1.1.1.284 | shoot apex | - |
Solanum lycopersicum | - |
1.1.1.284 | stamen | - |
Solanum lycopersicum | - |
1.1.1.284 | stem | - |
Solanum lycopersicum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.284 | additional information | the reaction mechanism involved the oxidation of a hydroxyl group of S-(hydroxymethyl)glutathione, spontaneous adduct of formaldehyde and glutathione, to form S-formylglutathione. Substrate specificity with alcohols and omega-hydroxyfatty acids, overview | Solanum lycopersicum | ? | - |
? | |
1.1.1.284 | additional information | the reaction mechanism involved the oxidation of a hydroxyl group of S-(hydroxymethyl)glutathione, spontaneous adduct of formaldehyde and glutathione, to form S-formylglutathione. Substrate specificity with alcohols and omega-hydroxyfatty acids, overview | Solanum lycopersicum Amateur | ? | - |
? | |
1.1.1.284 | S-(hydroxymethyl)glutathione + NAD+ | - |
Solanum lycopersicum | S-formylglutathione + NADH + H+ | - |
? | |
1.1.1.284 | S-(hydroxymethyl)glutathione + NAD+ | - |
Solanum lycopersicum Amateur | S-formylglutathione + NADH + H+ | - |
? | |
1.1.1.284 | S-nitrosoglutathione + NADH | - |
Solanum lycopersicum | ? + NAD+ | a variety of products depending on cellular conditions, including glutathione disulfide, glutathione sulfinamide and hydroxylamine | ? | |
1.1.1.284 | S-nitrosoglutathione + NADH | - |
Solanum lycopersicum Amateur | ? + NAD+ | a variety of products depending on cellular conditions, including glutathione disulfide, glutathione sulfinamide and hydroxylamine | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.284 | homodimer | 2 * 40000, SDS-PAGE | Solanum lycopersicum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.284 | GSNOR | - |
Solanum lycopersicum |
1.1.1.284 | HMGSH dehydrogenase | - |
Solanum lycopersicum |
1.1.1.284 | S-nitrosoglutathione reductase | - |
Solanum lycopersicum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.284 | 30 | - |
assay at | Solanum lycopersicum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.284 | 8 | - |
assay at | Solanum lycopersicum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.284 | 0.00043 | - |
N6022 | pH 8.0, 30°C, recombinant enzyme | Solanum lycopersicum | |
1.1.1.284 | 0.76 | - |
dodecanoic acid | pH 8.0, 30°C, recombinant enzyme | Solanum lycopersicum |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.1.1.284 | 0.0003 | - |
pH 8.0, 30°C, recombinant enzyme | Solanum lycopersicum | N6022 | |
1.1.1.284 | 0.526 | - |
pH 8.0, 30°C, recombinant enzyme | Solanum lycopersicum | dodecanoic acid | |
1.1.1.284 | 3.9 | - |
pH 8.0, 30°C, recombinant enzyme | Solanum lycopersicum | glutathione | |
1.1.1.284 | 5.1 | - |
pH 8.0, 30°C, recombinant enzyme | Solanum lycopersicum | Decanoic acid | |
1.1.1.284 | 5.8 | - |
pH 8.0, 30°C, recombinant enzyme | Solanum lycopersicum | S-Methylglutathione | |
1.1.1.284 | 6.1 | - |
pH 8.0, 30°C, recombinant enzyme | Solanum lycopersicum | octanoic acid |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.284 | evolution | the enzyme belongs to the large alcohol dehydrogenase superfamily, namely to the class III ADHs | Solanum lycopersicum |
1.1.1.284 | additional information | structure-function analysis, overview | Solanum lycopersicum |
1.1.1.284 | physiological function | in plants, GSNOR plays an important role in biotic and abiotic stress responses. S-nitrosylglutathione serves as a nitric oxide reservoir locally or possibly as NO donor in distant cells and tissues. NO and NO-related molecules such as S-nitrosothiols play a central role in the regulation of normal plant physiological processes and host defence. The key enzyme participates in the cellular homeostasis of S-NOs and in the metabolism of reactive nitrogen species | Solanum lycopersicum |