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Literature summary extracted from

  • Foumani, M.; Vuong, T.V.; Master, E.R.
    Altered substrate specificity of the gluco-oligosaccharide oxidase from Acremonium strictum (2011), Biotechnol. Bioeng., 108, 2261-2269.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.B3 expressed in Pichia pastoris strain GS115 Sarocladium strictum

Protein Variants

EC Number Protein Variants Comment Organism
1.1.99.B3 W351F the mutant shows reduced kcat values for monosaccharide and oligosaccharide substrates Sarocladium strictum
1.1.99.B3 Y300A the mutation doubles kcat values for monosaccharide and oligosaccharide substrates Sarocladium strictum
1.1.99.B3 Y300N the mutation doubles kcat values for monosaccharide and oligosaccharide substrates Sarocladium strictum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.B3 0.07
-
 cellobiose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.083
-
 cellobiose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.085
-
 cellotriose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.098
-
 xylobiose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.1
-
 xylotriose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.11
-
 cellotriose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.25
-
 cellobiose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.25
-
 cellotriose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.31
-
 xylotriose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.35
-
 xylobiose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.38
-
 cellobiose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.44
-
 cellotriose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 2.81
-
 maltose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 3.11
-
 D-glucose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 3.15
-
 xylotriose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 4.3
-
 xylotriose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 4.83
-
 xylobiose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 5
-
 maltose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 5.11
-
 xylobiose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 8.1
-
 D-glucose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 11
-
 maltose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 17.4
-
 D-glucose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 19.6
-
 maltose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 31
-
 D-glucose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 32
-
 D-xylose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 36.1
-
 D-galactose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 51.8
-
 D-xylose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 56
-
 N-acetyl-D-glucosamine mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 92
-
 N-acetyl-D-glucosamine mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 96
-
 D-galactose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 105
-
 D-xylose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 110
-
 D-galactose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 132
-
 D-galactose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 288
-
 D-xylose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 340
-
 N-acetyl-D-glucosamine wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 950
-
 N-acetyl-D-glucosamine mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.B3 cellobiose + O2 Sarocladium strictum
-
 ? + H2O2
-
 ?
1.1.99.B3 cellobiose + O2 Sarocladium strictum CBS 346.70
-
 ? + H2O2
-
 ?
1.1.99.B3 cellotriose + O2 Sarocladium strictum
-
 ? + H2O2
-
 ?
1.1.99.B3 cellotriose + O2 Sarocladium strictum CBS 346.70
-
 ? + H2O2
-
 ?
1.1.99.B3 D-galactose + O2 Sarocladium strictum
-
 ? + H2O2
-
 ?
1.1.99.B3 D-glucose + O2 Sarocladium strictum highest catalytic efficiency with D-glucose ? + H2O2
-
 ?
1.1.99.B3 D-xylose + O2 Sarocladium strictum
-
 ? + H2O2
-
 ?
1.1.99.B3 D-xylose + O2 Sarocladium strictum CBS 346.70
-
 ? + H2O2
-
 ?
1.1.99.B3 maltose + O2 Sarocladium strictum
-
 ? + H2O2
-
 ?
1.1.99.B3 maltose + O2 Sarocladium strictum CBS 346.70
-
 ? + H2O2
-
 ?
1.1.99.B3 additional information Sarocladium strictum no activity with mannose ?
-
 ?
1.1.99.B3 additional information Sarocladium strictum CBS 346.70 no activity with mannose ?
-
 ?
1.1.99.B3 N-acetyl-D-glucosamine + O2 Sarocladium strictum
-
 ? + H2O2
-
 ?
1.1.99.B3 xylobiose + O2 Sarocladium strictum
-
 ? + H2O2
-
 ?
1.1.99.B3 xylotriose + O2 Sarocladium strictum
-
 ? + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.B3 Sarocladium strictum Q6PW77
-
-
1.1.99.B3 Sarocladium strictum CBS 346.70 Q6PW77
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.B3 Ni-NTA resin column chromatography Sarocladium strictum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.99.B3 culture condition:wheat bran-grown cell
-
 Sarocladium strictum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.B3 cellobiose + O2
-
 Sarocladium strictum ? + H2O2
-
 ?
1.1.99.B3 cellobiose + O2
-
 Sarocladium strictum CBS 346.70 ? + H2O2
-
 ?
1.1.99.B3 cellotriose + O2
-
 Sarocladium strictum ? + H2O2
-
 ?
1.1.99.B3 cellotriose + O2
-
 Sarocladium strictum CBS 346.70 ? + H2O2
-
 ?
1.1.99.B3 D-galactose + O2
-
 Sarocladium strictum ? + H2O2
-
 ?
1.1.99.B3 D-glucose + O2 highest catalytic efficiency with D-glucose Sarocladium strictum ? + H2O2
-
 ?
1.1.99.B3 D-xylose + O2
-
 Sarocladium strictum ? + H2O2
-
 ?
1.1.99.B3 D-xylose + O2
-
 Sarocladium strictum CBS 346.70 ? + H2O2
-
 ?
1.1.99.B3 maltose + O2
-
 Sarocladium strictum ? + H2O2
-
 ?
1.1.99.B3 maltose + O2
-
 Sarocladium strictum CBS 346.70 ? + H2O2
-
 ?
1.1.99.B3 additional information no activity with mannose Sarocladium strictum ?
-
 ?
1.1.99.B3 additional information no activity with mannose Sarocladium strictum CBS 346.70 ?
-
 ?
1.1.99.B3 N-acetyl-D-glucosamine + O2
-
 Sarocladium strictum ? + H2O2
-
 ?
1.1.99.B3 xylobiose + O2
-
 Sarocladium strictum ? + H2O2
-
 ?
1.1.99.B3 xylotriose + O2
-
 Sarocladium strictum ? + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.B3 gluco-oligosaccharide oxidase
-
 Sarocladium strictum
1.1.99.B3 GOOX
-
 Sarocladium strictum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.B3 4.6
-
 D-xylose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 5.3
-
 cellotriose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 5.3
-
 D-xylose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 5.4
-
 maltose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 5.6
-
 D-glucose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 5.7
-
 cellobiose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 6
-
 maltose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 6
-
 cellotriose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 6.3
-
 cellobiose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 6.6
-
 D-galactose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 7.2
-
 D-galactose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 7.5
-
 D-glucose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 7.8
-
 N-acetyl-D-glucosamine mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 7.9
-
 xylotriose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 8
-
 xylobiose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 8.1
-
 N-acetyl-D-glucosamine wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 8.3
-
 xylotriose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 8.8
-
 xylobiose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 10
-
 cellotriose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 10.4
-
 maltose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 10.4
-
 maltose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 10.8
-
 D-glucose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 11.2
-
 cellotriose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 11.3
-
 D-xylose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 11.3
-
 N-acetyl-D-glucosamine mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 11.4
-
 cellobiose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 11.7
-
 D-xylose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 11.8
-
 D-galactose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 12
-
 xylobiose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 12
-
 xylotriose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 12.8
-
 N-acetyl-D-glucosamine mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 13.2
-
 D-glucose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 13.3
-
 D-galactose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 13.3
-
 xylobiose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 13.7
-
 cellobiose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 13.9
-
 xylotriose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.B3 0.008
-
 N-acetyl-D-glucosamine mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.02
-
 D-xylose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.02
-
 N-acetyl-D-glucosamine wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.05
-
 D-xylose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.06
-
 D-galactose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.11
-
 D-galactose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.14
-
 D-galactose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.14
-
 N-acetyl-D-glucosamine mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.18
-
 D-glucose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.18
-
 D-galactose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.2
-
 N-acetyl-D-glucosamine mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.22
-
 D-xylose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.36
-
 D-xylose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.43
-
 D-glucose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.53
-
 maltose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 0.95
-
 maltose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 1.1
-
 maltose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 1.63
-
 D-glucose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 2.13
-
 maltose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 2.48
-
 xylobiose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 2.6
-
 xylobiose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 2.83
-
 xylotriose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 3.6
-
 D-glucose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 4.33
-
 xylotriose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 23.3
-
 xylobiose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 23.3
-
 cellotriose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 25
-
 xylotriose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 30
-
 cellobiose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 45
-
 cellotriose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 48.3
-
 cellotriose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 56.7
-
 cellobiose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 68.3
-
 cellobiose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 70
-
 cellotriose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 85
-
 xylotriose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 90
-
 xylobiose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
1.1.99.B3 100
-
 cellobiose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum