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Literature summary extracted from

  • Honarbakhsh, M.; Villafane, A.A.; Ruhl, I.; Sannino, D.; Bini, E.
    Development of a thermostable beta-glucuronidase-based reporter system for monitoring gene expression in hyperthermophiles (2012), Biotechnol. Bioeng., 109, 1881-1886.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.31 analysis Sulfolobus gene can be used as reporter in any thermophilic microorganism lacking an endogenous beta-glucuronidase activity Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.31 expression in Escherichia coli. Although Escherichia coli possesses an endogenous beta-glucuronidase activity, a short treatment at 80°C of the crude extract causes the precipitation of most Escherichia coli protein, resulting in an enrichment of the Sulfolobus beta-glucuronidase Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.31 0.54
-
 4-methylumbelliferyl beta-D-glucuronide pH 6.5, 75°C Saccharolobus solfataricus
3.2.1.31 0.58
-
 4-methylumbelliferyl beta-D-glucuronide pH 6.5, 65°C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.31 66800
-
-
 Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.31 Saccharolobus solfataricus Q97UI1
-
-
3.2.1.31 Saccharolobus solfataricus P2 Q97UI1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.31
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.31 4-methylumbelliferyl beta-D-glucuronide + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + D-glucuronic acid
-
 ?
3.2.1.31 4-methylumbelliferyl beta-D-glucuronide + H2O
-
 Saccharolobus solfataricus P2 4-methylumbelliferone + D-glucuronic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.31 GusB
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.31 70 80
-
 Saccharolobus solfataricus
3.2.1.31 75
-
 assay at Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.31 37 100 37°C: about 40% of maximal activity, 100°C: about 50% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.31 65
-
 4.5 d, 1.1% loss of activity Saccharolobus solfataricus
3.2.1.31 70
-
 10 h, enzyme retains full activity Saccharolobus solfataricus
3.2.1.31 80
-
 10 h, enzyme retains full activity Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.31 4.5 6.5 highly active in the pH-range 4.5-6.5 Saccharolobus solfataricus
3.2.1.31 6.5
-
 assay at Saccharolobus solfataricus