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Literature summary extracted from

  • Graham, W.J. 5th; Rolfsmeier, M.L.; Haseltine, C.A.
    An archaeal RadA paralog influences presynaptic filament formation (2013), DNA Repair, 12, 403-413.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.4.B7 SsoRal1 protein in addition to constraining SsoRadA ssDNA-dependent ATPase activity, SsoRal1 enhances SsoRadA ssDNA binding, effectively influencing activities necessary for presynaptic filament formation. This results in enhanced SsoRadA-mediated strand invasion in the presence of SsoRal1 and suggests a filament stabilization function for the SsoRal1 protein Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
3.6.4.B7 K120A reduced ATPase activity, mutant K120A is able to bind ssDNA with ATP, ADP, or ATPgammaS under saturating protein conditions, but failed to bind well at subsaturating concentrations with ATP or ATPgammaS Saccharolobus solfataricus
3.6.4.B7 K120R reduced ATPase activity, mutant only binds ATP in the presence of ssDNA Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.6.4.B7 Saccharolobus solfataricus Q55075
-
-
3.6.4.B7 Saccharolobus solfataricus P2 Q55075
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.4.B7 mutant proteins K120A and K120R Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.4.B7 ATP + H2O the ATPase activity of SsoRadA is ssDNA-dependent. It is suggested that the recombinase first binds ATP, then binds DNA. ATP hydrolysis has no effect on ssDNA binding. After the protein is bound to ssDNA, it hydrolyzes ATP Saccharolobus solfataricus ADP + phosphate
-
 ?
3.6.4.B7 ATP + H2O the ATPase activity of SsoRadA is ssDNA-dependent. It is suggested that the recombinase first binds ATP, then binds DNA. ATP hydrolysis has no effect on ssDNA binding. After the protein is bound to ssDNA, it hydrolyzes ATP Saccharolobus solfataricus P2 ADP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.4.B7 SsoRadA
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.4.B7 80
-
 assay at Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.4.B7 7
-
 assay at Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
3.6.4.B7 physiological function recombinases of the RecA family play vital roles in homologous recombination, a high-fidelity mechanism to repair DNA double-stranded breaks. These proteins catalyze strand invasion and exchange after forming dynamic nucleoprotein filaments on ssDNA Saccharolobus solfataricus