BRENDA - Enzyme Database

A ternary mechanism for NADH oxidation by positively charged electron acceptors, catalyzed at the flavin site in respiratory complex I

Birrell, J.A.; King, M.S.; Hirst, J.; FEBS Lett. 585, 2318-2322 (2011)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
7.1.1.2
ADP-ribose
-
Bos taurus
7.1.1.2
ATP
-
Bos taurus
7.1.1.2
additional information
NADH, ATP, ADP and ADP-ribose stimulate the enzyme reactions, indicating that the positively-charged acceptors interact with their negatively charged phosphates. In contrast to direct O2 reduction the rate is stimulated, not inhibited, by high NADH concentrations. When NADH, ADP, ATP and ADP-ribose bind to the reduced flavin they stimulate paraquat and HAR reduction, but inhibit FeCN, APAD+ and O2 reduction. Adenosine, which lacks phosphates, does not stimulate the reaction with hexaammineruthenium(III), HAR, ternary mechanism of HAR and paraquat reduction,overview
Bos taurus
7.1.1.2
NADH
-
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
7.1.1.2
additional information
when NADH, ADP, ATP and ADP-ribose bind to the reduced flavin they stimulate paraquat and HAR reduction, but inhibit FeCN, APAD+ and O2 reduction
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.1.1.2
mitochondrial inner membrane
-
Bos taurus
5743
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.1.1.2
NADH + ubiquinone + 6 H+[side 1]
Bos taurus
-
NAD+ + ubiquinol + 7 H+[side 2]
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.1.1.2
Bos taurus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
7.1.1.2
heart
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.1.1.2
additional information
the positively-charged electron acceptors paraquat and hexaammineruthenium(III) react with the nucleotide-bound reduced flavin in complex I, by an unusual ternary mechanism, overview. The mechanism for paraquat reduction defines another mechanism for superoxide production by complex I by redox cycling
725003
Bos taurus
?
-
-
-
-
7.1.1.2
NADH + ubiquinone + 6 H+[side 1]
-
725003
Bos taurus
NAD+ + ubiquinol + 7 H+[side 2]
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
7.1.1.2
NADH
-
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
7.1.1.2
ADP-ribose
-
Bos taurus
7.1.1.2
ATP
-
Bos taurus
7.1.1.2
additional information
NADH, ATP, ADP and ADP-ribose stimulate the enzyme reactions, indicating that the positively-charged acceptors interact with their negatively charged phosphates. In contrast to direct O2 reduction the rate is stimulated, not inhibited, by high NADH concentrations. When NADH, ADP, ATP and ADP-ribose bind to the reduced flavin they stimulate paraquat and HAR reduction, but inhibit FeCN, APAD+ and O2 reduction. Adenosine, which lacks phosphates, does not stimulate the reaction with hexaammineruthenium(III), HAR, ternary mechanism of HAR and paraquat reduction,overview
Bos taurus
7.1.1.2
NADH
-
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
7.1.1.2
NADH
-
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
7.1.1.2
additional information
when NADH, ADP, ATP and ADP-ribose bind to the reduced flavin they stimulate paraquat and HAR reduction, but inhibit FeCN, APAD+ and O2 reduction
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.1.1.2
mitochondrial inner membrane
-
Bos taurus
5743
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.1.1.2
NADH + ubiquinone + 6 H+[side 1]
Bos taurus
-
NAD+ + ubiquinol + 7 H+[side 2]
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
7.1.1.2
heart
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.1.1.2
additional information
the positively-charged electron acceptors paraquat and hexaammineruthenium(III) react with the nucleotide-bound reduced flavin in complex I, by an unusual ternary mechanism, overview. The mechanism for paraquat reduction defines another mechanism for superoxide production by complex I by redox cycling
725003
Bos taurus
?
-
-
-
-
7.1.1.2
NADH + ubiquinone + 6 H+[side 1]
-
725003
Bos taurus
NAD+ + ubiquinol + 7 H+[side 2]
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
7.1.1.2
physiological function
the flavin mononucleotide in complex I catalyzes NADH oxidation, O2 reduction to superoxide, and the reduction of several artificial electron acceptors
Bos taurus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
7.1.1.2
physiological function
the flavin mononucleotide in complex I catalyzes NADH oxidation, O2 reduction to superoxide, and the reduction of several artificial electron acceptors
Bos taurus