EC Number | Cloned (Comment) | Organism |
---|---|---|
1.10.3.11 | genes cioA and cioB encoding subunits I and II, phylogenetic analysis | Gluconobacter oxydans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.11 | azide | - |
Gluconobacter oxydans | |
1.10.3.11 | additional information | CIO activity is much more resistant to cyanide, compared with Escherichia coli cytochrome bd, but sensitive to azide | Gluconobacter oxydans | |
1.10.3.11 | ubiquinol-1 | substrate inhibition | Gluconobacter oxydans | |
1.10.3.11 | ubiquinol-2 | substrate inhibition | Gluconobacter oxydans | |
1.10.3.11 | ubiquinone-1 | product inhibition, an excess amount of ubiquinol-2 is unable to suppress product inhibition with ubiquinone-1 therefore, the inhibition mode may not be competitive | Gluconobacter oxydans | |
1.10.3.11 | ubiquinone-2 | product inhibition | Gluconobacter oxydans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.11 | additional information | - |
additional information | Michaelis-Menten kinetics, overview | Gluconobacter oxydans | |
1.10.3.11 | 0.0729 | - |
ubiquinol-1 | pH 6.5, 35°C | Gluconobacter oxydans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.10.3.11 | membrane | outer surface of the cytoplasmic membrane | Gluconobacter oxydans | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.11 | Fe2+ | heme iron, 3.1 Fe2+ per enzyme molecule | Gluconobacter oxydans | |
1.10.3.11 | additional information | the enzyme contains no copper | Gluconobacter oxydans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.10.3.11 | 30000 | - |
x * 53000 + x * 30000, SDS-PAGE | Gluconobacter oxydans |
1.10.3.11 | 53000 | - |
x * 53000 + x * 30000, SDS-PAGE | Gluconobacter oxydans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.11 | 2 ubiquinol-1 + O2 | Gluconobacter oxydans | - |
2 ubiquinone-1 + 2 H2O | - |
? | |
1.10.3.11 | 2 ubiquinol-1 + O2 | Gluconobacter oxydans NBRC 3172 | - |
2 ubiquinone-1 + 2 H2O | - |
? | |
1.10.3.11 | 2 ubiquinol-10 + O2 | Gluconobacter oxydans | - |
2 ubiquinone-10 + 2 H2O | - |
? | |
1.10.3.11 | 2 ubiquinol-10 + O2 | Gluconobacter oxydans NBRC 3172 | - |
2 ubiquinone-10 + 2 H2O | - |
? | |
1.10.3.11 | 2 ubiquinol-2 + O2 | Gluconobacter oxydans | - |
2 ubiquinone-2 + 2 H2O | - |
? | |
1.10.3.11 | 2 ubiquinol-2 + O2 | Gluconobacter oxydans NBRC 3172 | - |
2 ubiquinone-2 + 2 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.10.3.11 | Gluconobacter oxydans | - |
subunit I, CioA; genes cioA and cioB encoding subunits I and II | - |
1.10.3.11 | Gluconobacter oxydans NBRC 3172 | - |
subunit I, CioA; genes cioA and cioB encoding subunits I and II | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.10.3.11 | native enzyme from membranes by detergent solubilization, anion exchange and hydroxyapatite chromatography | Gluconobacter oxydans |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.10.3.11 | 2 ubiquinol + O2 = 2 ubiquinone + 2 H2O | modified ping-pong bi-bi mechanism | Gluconobacter oxydans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.11 | 2 ubiquinol-1 + O2 | - |
Gluconobacter oxydans | 2 ubiquinone-1 + 2 H2O | - |
? | |
1.10.3.11 | 2 ubiquinol-1 + O2 | - |
Gluconobacter oxydans NBRC 3172 | 2 ubiquinone-1 + 2 H2O | - |
? | |
1.10.3.11 | 2 ubiquinol-10 + O2 | - |
Gluconobacter oxydans | 2 ubiquinone-10 + 2 H2O | - |
? | |
1.10.3.11 | 2 ubiquinol-10 + O2 | - |
Gluconobacter oxydans NBRC 3172 | 2 ubiquinone-10 + 2 H2O | - |
? | |
1.10.3.11 | 2 ubiquinol-2 + O2 | - |
Gluconobacter oxydans | 2 ubiquinone-2 + 2 H2O | - |
? | |
1.10.3.11 | 2 ubiquinol-2 + O2 | - |
Gluconobacter oxydans NBRC 3172 | 2 ubiquinone-2 + 2 H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.10.3.11 | ? | x * 53000 + x * 30000, SDS-PAGE | Gluconobacter oxydans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.10.3.11 | CIO | - |
Gluconobacter oxydans |
1.10.3.11 | cyanide-insensitive quinol oxidase | - |
Gluconobacter oxydans |
1.10.3.11 | cyanide-insensitive terminal quinol oxidase | - |
Gluconobacter oxydans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.11 | 25 | - |
assay at | Gluconobacter oxydans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.11 | 5 | 6 | - |
Gluconobacter oxydans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.11 | heme | the enzyme contains reduced hemes b and of oxygenated and ferric heme d, differing from cytochrome bd. Heme d serves as the ligand-binding site of CIO | Gluconobacter oxydans |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.10.3.11 | 0.82 | - |
pH 6.5, 25°C | Gluconobacter oxydans | azide |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.10.3.11 | evolution | the enzyme is a member of the subfamily of cytochrome bd present in bacterial respiratory chain, phylogenetic analysis | Gluconobacter oxydans |
1.10.3.11 | additional information | the purified CIO shows an extraordinary high ubiquinol-1 oxidase activity. The enzyme shows a modified ping-pong bi-bi mechanism | Gluconobacter oxydans |
1.10.3.11 | physiological function | ubiquinol-10 molecules are reoxidized by cytochrome bo3 and CIO, terminal oxidases of the respiratory chain. In the Gluconobacter oxydans respiratory chain, CIO may have a physiological role in compensation for lower activity of cytochrome bo3 under low growth pH to maintain rapid substrate oxidation | Gluconobacter oxydans |