EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.2.2 | 0.18 | - |
ferricyanide | S0.5 value, pH 4.0, 42°C | Campylobacter jejuni | |
1.8.2.2 | 0.2 | - |
thiosulfate | pH 4.0, 42°C | Campylobacter jejuni | |
1.8.99.B2 | 0.01 | - |
tetrathionate | pH 5.0, 42°C | Campylobacter jejuni |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.99.B2 | 36247 | - |
x * 36247, calculated from sequence | Campylobacter jejuni |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.99.B2 | tetrathionate + a reduced electron acceptor | Campylobacter jejuni | - |
2 thiosulfate + an oxidized electron acceptor | - |
? | |
1.8.99.B2 | tetrathionate + a reduced electron acceptor | Campylobacter jejuni NCTC 11828 | - |
2 thiosulfate + an oxidized electron acceptor | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.2.2 | Campylobacter jejuni | - |
bifunctional tetrathionate reductase/thiosulfate dehydrogenase | - |
1.8.2.2 | Campylobacter jejuni NCTC 11828 | - |
bifunctional tetrathionate reductase/thiosulfate dehydrogenase | - |
1.8.99.B2 | Campylobacter jejuni | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.99.B2 | - |
Campylobacter jejuni |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.8.99.B2 | 64 | - |
pH 5.0, 42°C | Campylobacter jejuni |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.2.2 | 2 thiosulfate + 2 ferricyanide | stoichiometric ratio of conversion is of 1 mole tetrathionate to 2 mol initial thiosulfate. Enzyme can couple thiosulfate oxidation to electron transport to oxygen | Campylobacter jejuni | tetrathionate + 2 ferrocyanide | - |
? | |
1.8.2.2 | 2 thiosulfate + 2 ferricyanide | stoichiometric ratio of conversion is of 1 mole tetrathionate to 2 mol initial thiosulfate. Enzyme can couple thiosulfate oxidation to electron transport to oxygen | Campylobacter jejuni NCTC 11828 | tetrathionate + 2 ferrocyanide | - |
? | |
1.8.99.B2 | tetrathionate + a reduced electron acceptor | - |
Campylobacter jejuni | 2 thiosulfate + an oxidized electron acceptor | - |
? | |
1.8.99.B2 | tetrathionate + a reduced electron acceptor | bifunctional tetrathionate reductase/thiosulphate dehydrogenase | Campylobacter jejuni | 2 thiosulfate + an oxidized electron acceptor | - |
? | |
1.8.99.B2 | tetrathionate + a reduced electron acceptor | - |
Campylobacter jejuni NCTC 11828 | 2 thiosulfate + an oxidized electron acceptor | - |
? | |
1.8.99.B2 | tetrathionate + a reduced electron acceptor | bifunctional tetrathionate reductase/thiosulphate dehydrogenase | Campylobacter jejuni NCTC 11828 | 2 thiosulfate + an oxidized electron acceptor | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.99.B2 | ? | x * 36247, calculated from sequence | Campylobacter jejuni |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.2.2 | C8J_0815 | locus name | Campylobacter jejuni |
1.8.2.2 | TsdA | - |
Campylobacter jejuni |
1.8.99.B2 | C8J_0815 | locus name | Campylobacter jejuni |
1.8.99.B2 | TsdA | bifunctional tetrathionate reductase/thiosulfate dehydrogenase | Campylobacter jejuni |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.2.2 | 42 | - |
- |
Campylobacter jejuni |
1.8.99.B2 | 42 | - |
assay at | Campylobacter jejuni |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.2.2 | 604 | - |
thiosulfate | pH 4.0, 42°C | Campylobacter jejuni | |
1.8.99.B2 | 426 | - |
tetrathionate | pH 5.0, 42°C | Campylobacter jejuni |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.2.2 | 4 | - |
substrate ferricyanide | Campylobacter jejuni |
1.8.99.B2 | 5 | - |
assay at | Campylobacter jejuni |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.2.2 | heme | sequence shows two Cys-X2-Cys-His heme c-binding motifs | Campylobacter jejuni | |
1.8.99.B2 | heme | sequence shows two Cys-X2-Cys-His heme c-binding motifs, bifunctional tetrathionate reductase/thiosulphate dehydrogenase | Campylobacter jejuni |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.8.2.2 | physiological function | growth of the microaerophilic mucosal pathogen Campylobacter jejuni under oxygen-limited conditions is stimulated by tetrathionate. A tsdA null mutant still slowly reduces, but can not grow on, tetrathionate under oxygen limitation, lacks thiosulfate-dependent respiration and fails to convert thiosulfate to tetrathionate microaerobically. Physiological role for Campylobacter jejuni TsdA is a tetrathionate reductase | Campylobacter jejuni |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.2.2 | 302 | - |
thiosulfate | pH 4.0, 42°C | Campylobacter jejuni | |
1.8.99.B2 | 42600 | - |
tetrathionate | pH 5.0, 42°C | Campylobacter jejuni |