Literature summary extracted from
Luo, D.; Niu, X.; Yu, J.; Yan, J.; Gou, X.; Lu, B.R.; Liu, Y.
Rice choline monooxygenase (OsCMO) protein functions in enhancing glycine betaine biosynthesis in transgenic tobacco but does not accumulate in rice (Oryza sativa L. ssp. japonica) (2012), Plant Cell Rep., 31, 1625-1635.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.15.7 |
gene OsCMO, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional OsCMO expression in transgenic Nicotiana tabacum cv. Xanthinc plants using Agrobacterium tumefaciens EHA105 for transfection, overexpressing plants show increased glycine betaine content and elevated tolerance to salt stress |
Oryza sativa |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.14.15.7 |
Fe2+ |
a mononuclear non-heme Fe2+, with binding sequence G/DX3-4DX2HX4-5H |
Oryza sativa |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.14.15.7 |
43000 |
- |
* 43000, recombinant enzyme, SDS-PAGE |
Oryza sativa |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.15.7 |
Oryza sativa |
B8B239 |
ssp. japonica, cv. Nipponbare, CMO single copy gene |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.15.7 |
choline + O2 + 2 reduced ferredoxin + 2 H+ |
- |
Oryza sativa |
betaine aldehyde hydrate + H2O + 2 oxidized ferredoxin |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.14.15.7 |
? |
* 43000, recombinant enzyme, SDS-PAGE |
Oryza sativa |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.15.7 |
Fe-S center |
Rieske-type [2Fe-2S] cluster sequence CXHX15-17CX2H |
Oryza sativa |
|
General Information
EC Number |
General Information |
Comment |
Organism |
---|
1.14.15.7 |
metabolism |
glycine betaine is a compatible quaternary amine that enables plants to tolerate abiotic stresses, including salt, drought and cold. In plants, glycine betaine is synthesized through two-step of successive oxidations from choline, catalyzed by choline monooxygenase and betaine aldehyde dehydrogenase, respectively. Oryza sativa is a typical non-glycine betaine accumulating species. The genome sequencing reveals orthologues of both choline monooxygenase and betaine aldehyde dehydrogenase, but, while the betaine aldehyde dehydrogenase is functional, the choline monooxygenase of japonica rice plant is not. Nevertheless the heterologously expressed rice enzyme is active in tabacco plants, overview |
Oryza sativa |