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Literature summary extracted from
- Overexpression of the coenzyme-F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase gene from the hyperthermophilic Methanopyrus kandleri (1997), Eur. J. Biochem., 245, 386-391.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.98.1 | (NH4)2SO4 | stimulates | Methanopyrus kandleri |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.5.98.1 | overexpression in Escherichia coli | Methanopyrus kandleri |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.98.1 | 0.02 | - |
5,10-methylenetetrahydromethanopterin | enzyme from Methanopyrus kandleri, pH and temperature not specified in the publication | Methanopyrus kandleri | |
| 1.5.98.1 | 0.03 | - |
5,10-methylenetetrahydromethanopterin | recombinant enzyme expressed in Escherichia coli, pH and temperature not specified in the publication | Methanopyrus kandleri | |
| 1.5.98.1 | 0.04 | - |
oxidized coenzyme F420 | recombinant enzyme expressed in Escherichia coli, pH and temperature not specified in the publication | Methanopyrus kandleri | |
| 1.5.98.1 | 0.08 | - |
oxidized coenzyme F420 | enzyme from Methanopyrus kandleri, pH and temperature not specified in the publication | Methanopyrus kandleri | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.5.98.1 | 31383 | - |
x * 31383, calculated from sequence | Methanopyrus kandleri |
| 1.5.98.1 | 34000 | - |
x * 34000, enzyme from Methanopyrus kandleri | Methanopyrus kandleri |
| 1.5.98.1 | 36000 | - |
x * 36000, recombinant enzyme expressed in Escherichia coli | Methanopyrus kandleri |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.98.1 | Methanopyrus kandleri | P94951 | - |
- |
| 1.5.98.1 | Methanopyrus kandleri DSM 6324 | P94951 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.98.1 | - |
Methanopyrus kandleri |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.98.1 | 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | - |
Methanopyrus kandleri | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? | |
| 1.5.98.1 | 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | - |
Methanopyrus kandleri DSM 6324 | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.98.1 | ? | x * 31383, calculated from sequence | Methanopyrus kandleri |
| 1.5.98.1 | ? | x * 34000, enzyme from Methanopyrus kandleri | Methanopyrus kandleri |
| 1.5.98.1 | ? | x * 36000, recombinant enzyme expressed in Escherichia coli | Methanopyrus kandleri |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.5.98.1 | coenzyme-F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase | - |
Methanopyrus kandleri |
| 1.5.98.1 | MTD | - |
Methanopyrus kandleri |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.98.1 | 65 | - |
assay at | Methanopyrus kandleri |
| 1.5.98.1 | 70 | 80 | - |
Methanopyrus kandleri |
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