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Literature summary extracted from

  • Jankowska, D.A.; Trautwein-Schult, A.; Cordes, A.; Hoferichter, P.; Klein, C.; Bode, R.; Baronian, K.; Kunze, G.
    Arxula adeninivorans xanthine oxidoreductase and its application in the production of food with low purine content (2013), J. Appl. Microbiol., 115, 796-807.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.3.2 0.0508
-
 hypoxanthine pH 8.5, 43°C Blastobotrys adeninivorans
1.17.3.2 0.0728
-
 xanthine pH 8.5, 43°C Blastobotrys adeninivorans
1.17.3.2 0.162
-
 NAD+ pH 8.5, 43°C Blastobotrys adeninivorans
1.17.3.2 0.208
-
 purine pH 8.5, 43°C Blastobotrys adeninivorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.3.2 Cd2+ inhibitory effect Blastobotrys adeninivorans
1.17.3.2 Cu2+ strong inhibition Blastobotrys adeninivorans
1.17.3.2 Fe2+ inhibitory effect Blastobotrys adeninivorans
1.17.3.2 Zn2+ inhibitory effect Blastobotrys adeninivorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.3.2 160000
-
 SDS-PAGE Blastobotrys adeninivorans
1.17.3.2 360000
-
 gel filtration Blastobotrys adeninivorans

Organism

EC Number Organism UniProt Comment Textmining
1.17.3.2 Blastobotrys adeninivorans R4ZGN4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.3.2 hypoxanthine + NAD+ + H2O
-
 Blastobotrys adeninivorans xanthine + NADH + H+
-
 ?
1.17.3.2 pterine + NAD+
-
 Blastobotrys adeninivorans ? + H2O + NADH
-
 ?
1.17.3.2 purine + NAD+
-
 Blastobotrys adeninivorans ? + H2O + NADH
-
 ?
1.17.3.2 xanthine + H2O + O2
-
 Blastobotrys adeninivorans urate + H2O2
-
 ?
1.17.3.2 xanthine + NAD+ + H2O
-
 Blastobotrys adeninivorans urate + NADH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.3.2 AXOR
-
 Blastobotrys adeninivorans
1.17.3.2 xanthine oxidoreductase
-
 Blastobotrys adeninivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.3.2 43
-
-
 Blastobotrys adeninivorans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.17.3.2 35 50
-
 Blastobotrys adeninivorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.3.2 30
-
 5% activity in 1 h at 30°C Blastobotrys adeninivorans
1.17.3.2 40
-
 12% activity in 1 h at 40°C Blastobotrys adeninivorans
1.17.3.2 50
-
 22% activity in 1 h at 50°C Blastobotrys adeninivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.3.2 8.5
-
 glycine-NaOH buffer Blastobotrys adeninivorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.17.3.2 7.5 9.5 potassium phosphate, Tris-HCl, Tris-acetate and glycine-NaOH buffer Blastobotrys adeninivorans

General Information

EC Number General Information Comment Organism
1.17.3.2 malfunction deletion mutant exhibits less than 1% of the wild-type strain activity towards xanthine and hypoxanthine under inducible conditions and both strains show no activity after cultivation without inducer (adenine) Blastobotrys adeninivorans