Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhu, X.; Li, T.; Gu, X.; Zhang, S.; Liu, Y.; Wang, Y.; Tan, X.
    Structural and functional investigation into acetyl-coenzyme A synthase and methyltransferase from human pathogen Clostridium difficile (2013), Metallomics, 5, 551-558.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.258 expression in Escherichia coli Clostridioides difficile
2.3.1.169 expression in Escherichia coli Clostridioides difficile

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.169 1,10-phenanthroline 1 mM, complete inhibition. 1,10-Phenanthroline additionally has inhibitory effects on growth of Peptoclostridium difficile Clostridioides difficile
2.3.1.169 2,2-dipyridyl 1 mM, complete inhibition. 2,2-Dipyridyl additionally has inhibitory effects on growth of Peptoclostridium difficile Clostridioides difficile

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.258 0.0178
-
 Cobalamin 25°C, pH 5.1 Clostridioides difficile
2.1.1.258 0.06
-
 Cobalamin 25°C, pH 7.2 Clostridioides difficile

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.169 Iron protein contains in average 3.7 Fe atoms and 1.6 Ni atoms per monomer molecule, which is consistent with the presence of a [NipNid]-[Fe4S4]-center Clostridioides difficile
2.3.1.169 Nickel protein contains in average 3.7 Fe atoms and 1.6 Ni atoms per monomer molecule, which is consistent with the presence of a [NipNid]-[Fe4S4]-center Clostridioides difficile

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.258 Clostridioides difficile
-
-
-
2.1.1.258 Clostridioides difficile 630
-
-
-
2.3.1.169 Clostridioides difficile
-
-
-
2.3.1.169 Clostridioides difficile 630
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.258 cobalamin + 5-methyltetrahydrofolate reaction is pH dependent with the best pH of around 5.0 Clostridioides difficile methylcobalamin + tetrahydrofolate
-
 ?
2.1.1.258 cobalamin + 5-methyltetrahydrofolate reaction is pH dependent with the best pH of around 5.0 Clostridioides difficile 630 methylcobalamin + tetrahydrofolate
-
 ?
2.3.1.169 CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA
-
 Clostridioides difficile CH3-CO-S-CoA + corrinoid/iron-sulfur protein
-
 ?
2.3.1.169 CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA
-
 Clostridioides difficile 630 CH3-CO-S-CoA + corrinoid/iron-sulfur protein
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.258 2.63
-
 Cobalamin 25°C, pH 5.1 Clostridioides difficile
2.1.1.258 37.2
-
 Cobalamin 25°C, pH 7.2 Clostridioides difficile

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.169 [4Fe-4S]-center protein contains in average 3.7 Fe atoms and 1.6 Ni atoms per monomer molecule, which is consistent with the presence of a [NipNid] [Fe4S4] center. Preparation shows a broad absorption at the 420 nm region Clostridioides difficile

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.258 Clostridioides difficile calculated
-
 4.8

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.258 150
-
 Cobalamin 25°C, pH 5.1 Clostridioides difficile
2.1.1.258 620
-
 Cobalamin 25°C, pH 7.2 Clostridioides difficile
2.3.1.169 0.66
-
 HS-CoA at 1 atm CO, pH not specified in the publication, temperature not specified in the publication Clostridioides difficile