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Literature summary extracted from

  • Yamanishi, M.; Kinoshita, K.; Fukuoka, M.; Saito, T.; Tanokuchi, A.; Ikeda, Y.; Obayashi, H.; Mori, K.; Shibata, N.; Tobimatsu, T.; Toraya, T.
    Redesign of coenzyme B12 dependent diol dehydratase to be resistant to the mechanism-based inactivation by glycerol and act on longer chain 1,2-diols (2012), FEBS J., 279, 793-804.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.28 expressed in Escherichia coli JM109 cells Klebsiella oxytoca

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.28 complexed with cyanocobalamin and glycerol, sandwich-drop vapor diffusion method, using 20 mM Tris/HCl buffer (pH 8.0) containing 14%-18% (w/v) poly(ethylene glycol) 6000, 0.23-0.25 M ammonium sulfate and 0.2% lauryl dimethylamine oxide Klebsiella oxytoca

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.28 Q336A the mutant shows decreased activity (21%) compared to the wild type enzyme Klebsiella oxytoca
4.2.1.28 S301A the mutant shows decreased activity (76%) compared to the wild type enzyme Klebsiella oxytoca
4.2.1.28 S301A/Q336A the mutant shows lowest activity (9.8%) compared to the wild type enzyme Klebsiella oxytoca

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.28 glycerol
-
 Klebsiella oxytoca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.28 0.11
-
 (S)-propane-1,2-diol wild type enzyme, at pH 8.0 and 37°C Klebsiella oxytoca
4.2.1.28 0.26
-
 (S)-propane-1,2-diol mutant enzyme S301A, at pH 8.0 and 37°C Klebsiella oxytoca
4.2.1.28 0.47
-
 (R)-Propane-1,2-diol wild type enzyme, at pH 8.0 and 37°C Klebsiella oxytoca
4.2.1.28 0.66
-
 (S)-propane-1,2-diol mutant enzyme Q336A, at pH 8.0 and 37°C Klebsiella oxytoca
4.2.1.28 0.7
-
 (R)-Propane-1,2-diol mutant enzyme S301A, at pH 8.0 and 37°C Klebsiella oxytoca
4.2.1.28 2.1
-
 (R)-Propane-1,2-diol mutant enzyme Q336A, at pH 8.0 and 37°C Klebsiella oxytoca
4.2.1.28 2.6
-
 (S)-propane-1,2-diol mutant enzyme S301A/Q336A, at pH 8.0 and 37°C Klebsiella oxytoca
4.2.1.28 3.9
-
 (R)-Propane-1,2-diol mutant enzyme S301A/Q336A, at pH 8.0 and 37°C Klebsiella oxytoca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.28 (R)-propane-1,2-diol Klebsiella oxytoca
-
 propanal + H2O
-
 ?
4.2.1.28 (R)-propane-1,2-diol Klebsiella oxytoca ATCC 8724
-
 propanal + H2O
-
 ?
4.2.1.28 (S)-propane-1,2-diol Klebsiella oxytoca
-
 propanal + H2O
-
 ?
4.2.1.28 (S)-propane-1,2-diol Klebsiella oxytoca ATCC 8724
-
 propanal + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.28 Klebsiella oxytoca Q59470
-
-
4.2.1.28 Klebsiella oxytoca ATCC 8724 Q59470
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.28 DEAE-cellulose column chromatography Klebsiella oxytoca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.28 (R)-propane-1,2-diol
-
 Klebsiella oxytoca propanal + H2O
-
 ?
4.2.1.28 (R)-propane-1,2-diol
-
 Klebsiella oxytoca ATCC 8724 propanal + H2O
-
 ?
4.2.1.28 (S)-propane-1,2-diol
-
 Klebsiella oxytoca propanal + H2O
-
 ?
4.2.1.28 (S)-propane-1,2-diol
-
 Klebsiella oxytoca ATCC 8724 propanal + H2O
-
 ?
4.2.1.28 butane-1,2-diol 107% activity compared to propane-1,2-diol Klebsiella oxytoca ?
-
 ?
4.2.1.28 butane-1,2-diol 107% activity compared to propane-1,2-diol Klebsiella oxytoca ATCC 8724 ?
-
 ?
4.2.1.28 additional information no activity with hexane-1,2-diol Klebsiella oxytoca ?
-
 ?
4.2.1.28 additional information no activity with hexane-1,2-diol Klebsiella oxytoca ATCC 8724 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.28 diol dehydratase alpha subunit
-
 Klebsiella oxytoca

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.28 coenzyme B12 dependent on Klebsiella oxytoca