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Literature summary extracted from

  • Richardson, J.S.; Carpena, X.; Switala, J.; Perez-Luque, R.; Donald, L.J.; Loewen, P.C.; Oresnik, I.J.
    RhaU of Rhizobium leguminosarum is a rhamnose mutarotase (2008), J. Bacteriol., 190, 2903-2910.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.32 expresssion in Escherichia coli Rhizobium leguminosarum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.3.32 to 1.6 A resolution. Structure shows a dimer in the asymmetric unit with a very similar structure to that of L-rhamnose mutarotase YiiL of Escherichia coli Rhizobium leguminosarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.32 12000
-
 2 * 12000 and 2 * 14000, SDS-PAGE, 2 * 16595, mass spectrometry, 2 * 16595,calculated Rhizobium leguminosarum
5.1.3.32 14000
-
 2 * 12000 and 2 * 14000, SDS-PAGE, 2 * 16595, mass spectrometry, 2 * 16595,calculated Rhizobium leguminosarum
5.1.3.32 16595
-
 2 * 12000 and 2 * 14000, SDS-PAGE, 2 * 16595, mass spectrometry, 2 * 16595,calculated Rhizobium leguminosarum
5.1.3.32 28000
-
 SDS-PAGE Rhizobium leguminosarum

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.32 Rhizobium leguminosarum Q7BSH1
-
-

Subunits

EC Number Subunits Comment Organism
5.1.3.32 dimer 2 * 12000 and 2 * 14000, SDS-PAGE, 2 * 16595, mass spectrometry, 2 * 16595,calculated Rhizobium leguminosarum
5.1.3.32 More mass spectrometry hows that enzyme exists predominantly as a dimer and that increasing ionization voltage causes its dissociation to a monomer Rhizobium leguminosarum

Synonyms

EC Number Synonyms Comment Organism
5.1.3.32 RhaU
-
 Rhizobium leguminosarum

General Information

EC Number General Information Comment Organism
5.1.3.32 physiological function growth phenotype on L-rhamnose of a a gene deletion strain is slower than that of the wild-type strain, although the ultimate cell yields are equivalent. The transport of L-rhamnose into the cell and the rate of its phosphorylation are unaffected by the deletion Rhizobium leguminosarum