Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ploier, B.; Scharwey, M.; Koch, B.; Schmidt, C.; Schatte, J.; Rechberger, G.; Kollroser, M.; Hermetter, A.; Daum, G.
    Screening for hydrolytic enzymes reveals Ayr1p as a novel triacylglycerol lipase in Saccharomyces cerevisiae (2013), J. Biol. Chem., 288, 36061-36072.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.3 S18A the mutation completely abolishes lipase activity Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 1.5 2 4-nitrophenyl butyrate at pH 7.4 and 30°C Saccharomyces cerevisiae
3.1.1.3 3.5
-
 4-nitrophenyl acetate at pH 7.4 and 30°C Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 60000
-
 x * 60000, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.3 triacylglycerol + H2O Saccharomyces cerevisiae
-
 diacylglycerol + a carboxylate
-
 ?
3.1.1.3 triacylglycerol + H2O Saccharomyces cerevisiae TM
-
 diacylglycerol + a carboxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Saccharomyces cerevisiae
-
-
-
3.1.1.3 Saccharomyces cerevisiae TM
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl acetate + H2O
-
 Saccharomyces cerevisiae 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl acetate + H2O
-
 Saccharomyces cerevisiae TM 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Saccharomyces cerevisiae 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Saccharomyces cerevisiae TM 4-nitrophenol + butyrate
-
 ?
3.1.1.3 triacylglycerol + H2O
-
 Saccharomyces cerevisiae diacylglycerol + a carboxylate
-
 ?
3.1.1.3 triacylglycerol + H2O
-
 Saccharomyces cerevisiae TM diacylglycerol + a carboxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 60000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 Ayr1p
-
 Saccharomyces cerevisiae
3.1.1.3 TG lipase
-
 Saccharomyces cerevisiae