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Literature summary extracted from

  • Hopkinson, B.M.; Meile, C.; Shen, C.
    Quantification of extracellular carbonic anhydrase activity in two marine diatoms and investigation of its role (2013), Plant Physiol., 162, 1142-1152.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 acetazolamide at very low CO2 concentrations enzyme inhibition reduces photosynthesis Conticribra weissflogii
4.2.1.1 acetazolamide at very low CO2 concentrations enzyme inhibition reduces photosynthesis Thalassiosira pseudonana
4.2.1.1 dextran-bound acetazolamide at very low CO2 concentrations enzyme inhibition reduces photosynthesis. Inhibition of enzyme reduces growth by about 10% at pH 8.4 Conticribra weissflogii
4.2.1.1 dextran-bound acetazolamide at very low CO2 concentrations enzyme inhibition reduces photosynthesis Thalassiosira pseudonana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 0.087
-
 CO2 pH 7.9, temperature not specified in the publication Conticribra weissflogii

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Conticribra weissflogii
-
-
-
4.2.1.1 Thalassiosira pseudonana
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 CO2 + H2O
-
 Conticribra weissflogii H2CO3
-
 ?
4.2.1.1 CO2 + H2O
-
 Thalassiosira pseudonana H2CO3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 eCA
-
 Conticribra weissflogii
4.2.1.1 eCA
-
 Thalassiosira pseudonana
4.2.1.1 extracellular carbonic anhydrase
-
 Conticribra weissflogii
4.2.1.1 extracellular carbonic anhydrase
-
 Thalassiosira pseudonana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.1 150
-
 CO2 pH 7.9, temperature not specified in the publication, the activities depended on culture conditions, ranging between 20/sec and 150/sec Conticribra weissflogii
4.2.1.1 200
-
 CO2 pH 7.9, temperature not specified in the publication, the activities depended on culture conditions, ranging between 80/sec and 200/sec Thalassiosira pseudonana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.1 7.9
-
 assay at Conticribra weissflogii
4.2.1.1 7.9
-
 assay at Thalassiosira pseudonana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.1 7.5 8.4 enzyme activity increases linearly with pH Conticribra weissflogii
4.2.1.1 7.8 8.6 enzyme activity increases linearly with pH Thalassiosira pseudonana

Expression

EC Number Organism Comment Expression
4.2.1.1 Thalassiosira pseudonana eCA expression is primarily controlled by CO2 up

General Information

EC Number General Information Comment Organism
4.2.1.1 malfunction at very low CO2 concentrations eCA inhibition reduces photosynthesis Thalassiosira pseudonana
4.2.1.1 malfunction at very low CO2 concentrations enzyme inhibition reduces photosynthesis Conticribra weissflogii