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Literature summary extracted from

  • Sakamoto, T.; Nishimura, Y.; Makino, Y.; Sunagawa, Y.; Harada, N.
    Biochemical characterization of a GH53 endo-beta-1,4-galactanase and a GH35 exo-beta-1,4-galactanase from Penicillium chrysogenum (2013), Appl. Microbiol. Biotechnol., 97, 2895-2906.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.89 gene Pcgal1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain BL21, subcloning in Escherichia coli strain DH5alpha Penicillium chrysogenum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.89 extracellular the enzyme is secreted Penicillium chrysogenum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.89 38000
-
 x * 38000, native enzyme, SDS-PAGE, x * 78000, recombinant enzyme, SDS-PAGE Penicillium chrysogenum
3.2.1.89 78000
-
 x * 38000, native enzyme, SDS-PAGE, x * 78000, recombinant enzyme, SDS-PAGE Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.89 Penicillium chrysogenum B5MGR3 gene Pcgal1
-
3.2.1.89 Penicillium chrysogenum 31B B5MGR3 gene Pcgal1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.89 native extracellular enzyme from the culture supernatant by ammonium sulfate fractionation, ultrafiltration, anion exchange and hydrophobic interaction chromatography, and gel filtration Penicillium chrysogenum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.89 7.6
-
 purified recombinant enzyme, substrate lupin galactan, pH 5.0, 37°C Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.89 debranched sugar beet arabinan + H2O low activity Penicillium chrysogenum ?
-
 ?
3.2.1.89 debranched sugar beet arabinan + H2O low activity Penicillium chrysogenum 31B ?
-
 ?
3.2.1.89 lupin beta-1,4-galactan + H2O best substrate Penicillium chrysogenum ?
-
 ?
3.2.1.89 lupin beta-1,4-galactan + H2O best substrate Penicillium chrysogenum 31B ?
-
 ?
3.2.1.89 additional information substrate specificity, overview. The smallest substrate for the enzyme is beta-1,4-galactotriose. No activity with sugar beet pectin, beta-1,3-galactan from larch wood, and soybean rhamnogalacturonan. No activity with 4-nitrophenly beta-D-galactopyranoside Penicillium chrysogenum ?
-
 ?
3.2.1.89 additional information substrate specificity, overview. The smallest substrate for the enzyme is beta-1,4-galactotriose. No activity with sugar beet pectin, beta-1,3-galactan from larch wood, and soybean rhamnogalacturonan. No activity with 4-nitrophenly beta-D-galactopyranoside Penicillium chrysogenum 31B ?
-
 ?
3.2.1.89 soybean arabinogalactan + H2O high activity Penicillium chrysogenum ?
-
 ?
3.2.1.89 soybean arabinogalactan + H2O high activity Penicillium chrysogenum 31B ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.89 ? x * 38000, native enzyme, SDS-PAGE, x * 78000, recombinant enzyme, SDS-PAGE Penicillium chrysogenum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.89 endo-beta-1,4-galactanase
-
 Penicillium chrysogenum
3.2.1.89 PcGAL1
-
 Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.89 40
-
-
 Penicillium chrysogenum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.89 40
-
 purified recombinant enzyme, pH 5.0, over 80% activity remaining after 1 h Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.89 5
-
-
 Penicillium chrysogenum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.89 3 9 purified recombinant enzyme, over 80% activity remaining after 16 h Penicillium chrysogenum

General Information

EC Number General Information Comment Organism
3.2.1.89 evolution the enzyme belongs to the glycosyl hydrolase family 53, GH53 Penicillium chrysogenum
3.2.1.89 additional information the enzyme acts synergistically with the exo-beta-1,4-galactanase of Penicillium chrysogenum Penicillium chrysogenum