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Literature summary extracted from

  • Johnston, J.B.; Kells, P.M.; Podust, L.M.; Ortiz de Montellano, P.R.
    Biochemical and structural characterization of CYP124: a methyl-branched lipid omega-hydroxylase from Mycobacterium tuberculosis (2009), Proc. Natl. Acad. Sci. USA, 106, 20687-20692.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.14 expressed in Escherichia coli DH5alpha cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.15.14 hanging drop vapor diffusion method, free enzyme using 1% (w/v) PEGMME 2000, 0.1 M Na cacodylate (pH 6.8), and 0.9 M succinic acid, and enzyme in complex with phytanic acid using 21% (w/v) PEG 4000, 0.1 M Bis-Tris (pH 5.5), and 0.2 M calcium acetate Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.14 0.009
-
 15-methylpalmitic acid at pH 7.4 and 25°C Mycobacterium tuberculosis
1.14.15.14 0.032
-
 geranylgeraniol at pH 7.4 and 25°C Mycobacterium tuberculosis
1.14.15.14 0.036
-
 farnesol at pH 7.4 and 25°C Mycobacterium tuberculosis
1.14.15.14 0.054
-
 phytanic acid at pH 7.4 and 25°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.14 48700
-
 x * 48700, calculated from amino acid sequence Mycobacterium tuberculosis
1.14.15.14 50000
-
 x * 50000, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.14 a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ Mycobacterium tuberculosis
-
 an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
 ?
1.14.15.14 a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ Mycobacterium tuberculosis H37Rv
-
 an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.14 Mycobacterium tuberculosis P9WPP3
-
-
1.14.15.14 Mycobacterium tuberculosis H37Rv P9WPP3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.14 Ni2+-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.14 15-methylpalmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
 Mycobacterium tuberculosis ?
-
 ?
1.14.15.14 15-methylpalmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
1.14.15.14 a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
 Mycobacterium tuberculosis an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
 ?
1.14.15.14 a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
 Mycobacterium tuberculosis H37Rv an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
 ?
1.14.15.14 farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ best substrate Mycobacterium tuberculosis ?
-
 ?
1.14.15.14 farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ best substrate Mycobacterium tuberculosis H37Rv ?
-
 ?
1.14.15.14 farnesyl diphosphate + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
 Mycobacterium tuberculosis ?
-
 ?
1.14.15.14 farnesyl diphosphate + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
1.14.15.14 geranylgeraniol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
 Mycobacterium tuberculosis ?
-
 ?
1.14.15.14 additional information the enzyme shows no activity with geraniol, lauric acid, phytane, pristane, phylloquinone (vitamin K1), menaquinone (vitamin K2), or bifonazole Mycobacterium tuberculosis ?
-
 ?
1.14.15.14 additional information the enzyme shows no activity with geraniol, lauric acid, phytane, pristane, phylloquinone (vitamin K1), menaquinone (vitamin K2), or bifonazole Mycobacterium tuberculosis H37Rv ?
-
 ?
1.14.15.14 palmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ the enzyme oxidizes the omega-1,omega-2, and omega-position of palmitic acid Mycobacterium tuberculosis ?
-
 ?
1.14.15.14 phytanic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
 Mycobacterium tuberculosis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.14 ? x * 50000, SDS-PAGE Mycobacterium tuberculosis
1.14.15.14 ? x * 48700, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.14.15.14 CYP124
-
 Mycobacterium tuberculosis