EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.2 | expression in Escherichia coli | Pectobacterium atrosepticum |
1.1.1.71 | expressed in Escherichia coli M15[pREP4] cells | Pectobacterium atrosepticum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.2 | D194A | mutation in putative metal-coordinating residue, almost complete loss of activity | Pectobacterium atrosepticum |
1.1.1.2 | H198A | mutation in putative metal-coordinating residue, almost complete loss of activity | Pectobacterium atrosepticum |
1.1.1.2 | H267A | mutation in putative metal-coordinating residue, almost complete loss of activity | Pectobacterium atrosepticum |
1.1.1.2 | H281A | mutation in putative metal-coordinating residue, almost complete loss of activity | Pectobacterium atrosepticum |
1.1.1.2 | H363A | 107% of wild-type activity | Pectobacterium atrosepticum |
1.1.1.71 | D194A | the mutation results in substantial catalytic deficiency (2.8% compared to the wild type enzyme) | Pectobacterium atrosepticum |
1.1.1.71 | H198A | inactive | Pectobacterium atrosepticum |
1.1.1.71 | H267A | inactive | Pectobacterium atrosepticum |
1.1.1.71 | H281A | inactive | Pectobacterium atrosepticum |
1.1.1.71 | H363A | the mutant with full activity is not able to grow on butanal-containing medium | Pectobacterium atrosepticum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.2 | EDTA | 0.1 mM, 38% residual activity. Activity is completely restored by the addition of 1 mM Co2+ and to 37% by addition of Ni2+, respectively | Pectobacterium atrosepticum | |
1.1.1.2 | NADPH | strong inhibition at high NADPH concentrations, assay below 0.8 mM | Pectobacterium atrosepticum | |
1.1.1.71 | EDTA | complete inhibition at 1 mM | Pectobacterium atrosepticum | |
1.1.1.71 | Fe2+ | no activity at 1 mM | Pectobacterium atrosepticum | |
1.1.1.71 | Ni2+ | 14% residual activity at 1 mM | Pectobacterium atrosepticum | |
1.1.1.71 | Zn2+ | 14% residual activity at 1 mM | Pectobacterium atrosepticum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.2 | 0.1 | - |
NADPH | pH 7.0, temperature not specified in the publication | Pectobacterium atrosepticum | |
1.1.1.2 | 0.4 | - |
pentanal | pH 7.0, temperature not specified in the publication | Pectobacterium atrosepticum | |
1.1.1.2 | 6.8 | - |
Butanal | pH 7.0, temperature not specified in the publication | Pectobacterium atrosepticum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.71 | Co2+ | required | Pectobacterium atrosepticum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 42000 | - |
- |
Pectobacterium atrosepticum |
1.1.1.2 | 42100 | - |
- |
Pectobacterium atrosepticum |
1.1.1.2 | 71000 | - |
gel filtration | Pectobacterium atrosepticum |
1.1.1.71 | 42100 | - |
- |
Pectobacterium atrosepticum |
1.1.1.71 | 43200 | - |
- |
Pectobacterium atrosepticum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.2 | Pectobacterium atrosepticum | U6CL97 | - |
- |
1.1.1.2 | Pectobacterium atrosepticum DSM 18077 | U6CL97 | - |
- |
1.1.1.71 | Pectobacterium atrosepticum | U6CL97 | - |
- |
1.1.1.71 | Pectobacterium atrosepticum DSM18077 | U6CL97 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.71 | Ni-NTA column chromatography and Superdex 200 pg gel filtration | Pectobacterium atrosepticum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.2 | butanal + NADPH + H+ | - |
Pectobacterium atrosepticum | butan-1-ol + NADP+ | - |
? | |
1.1.1.2 | butanal + NADPH + H+ | - |
Pectobacterium atrosepticum DSM18077 | butan-1-ol + NADP+ | - |
? | |
1.1.1.2 | butanal + NADPH + H+ | - |
Pectobacterium atrosepticum DSM 18077 | butan-1-ol + NADP+ | - |
? | |
1.1.1.2 | additional information | enzyme does not catalyze the oxidation of alcoholic substrates butanol, propanol, ethanol, methanol, glycerol, 1,3-propanediol, and 1,2-propandiol with NAD(P)+ as cofactor | Pectobacterium atrosepticum | ? | - |
? | |
1.1.1.2 | additional information | enzyme does not catalyze the oxidation of alcoholic substrates butanol, propanol, ethanol, methanol, glycerol, 1,3-propanediol, and 1,2-propandiol with NAD(P)+ as cofactor | Pectobacterium atrosepticum DSM18077 | ? | - |
? | |
1.1.1.2 | additional information | enzyme does not catalyze the oxidation of alcoholic substrates butanol, propanol, ethanol, methanol, glycerol, 1,3-propanediol, and 1,2-propandiol with NAD(P)+ as cofactor | Pectobacterium atrosepticum DSM 18077 | ? | - |
? | |
1.1.1.2 | pentanal + NADPH + H+ | - |
Pectobacterium atrosepticum | pentan-1-ol + NADP+ | - |
? | |
1.1.1.2 | pentanal + NADPH + H+ | - |
Pectobacterium atrosepticum DSM18077 | pentan-1-ol + NADP+ | - |
? | |
1.1.1.2 | pentanal + NADPH + H+ | - |
Pectobacterium atrosepticum DSM 18077 | pentan-1-ol + NADP+ | - |
? | |
1.1.1.71 | butanal + NADPH + H+ | 44% activity compared to pentanal | Pectobacterium atrosepticum | 1-butanol + NAD(P)+ | - |
? | |
1.1.1.71 | furfural + NADPH + H+ | 5% activity compared to pentanal | Pectobacterium atrosepticum | (furan-2-yl)methanol + NAD(P)+ | - |
? | |
1.1.1.71 | glycoaldehyde + NADPH + H+ | 5% activity compared to pentanal | Pectobacterium atrosepticum | ? | - |
? | |
1.1.1.71 | heptanal + NADPH + H+ | 56% activity compared to pentanal | Pectobacterium atrosepticum | 1-heptanol + NAD(P)+ | - |
? | |
1.1.1.71 | hexanal + NADPH + H+ | 62% activity compared to pentanal | Pectobacterium atrosepticum | 1-hexanol + NAD(P)+ | - |
? | |
1.1.1.71 | methylglyoxal + NADPH + H+ | 29% activity compared to pentanal | Pectobacterium atrosepticum | ? | - |
? | |
1.1.1.71 | additional information | no activity with glyoxal, methanal, and ethanal | Pectobacterium atrosepticum | ? | - |
? | |
1.1.1.71 | additional information | the enzyme shows no oxidative activities towards alcoholic compounds | Pectobacterium atrosepticum | ? | - |
? | |
1.1.1.71 | additional information | no activity with glyoxal, methanal, and ethanal | Pectobacterium atrosepticum DSM18077 | ? | - |
? | |
1.1.1.71 | additional information | the enzyme shows no oxidative activities towards alcoholic compounds | Pectobacterium atrosepticum DSM18077 | ? | - |
? | |
1.1.1.71 | additional information | no activity with glyoxal, methanal, and ethanal | Pectobacterium atrosepticum DSM 18077 | ? | - |
? | |
1.1.1.71 | additional information | the enzyme shows no oxidative activities towards alcoholic compounds | Pectobacterium atrosepticum DSM 18077 | ? | - |
? | |
1.1.1.71 | octanal + NADPH + H+ | 55% activity compared to pentanal | Pectobacterium atrosepticum | 1-octanol + NAD(P)+ | - |
? | |
1.1.1.71 | pentanal + NADPH + H+ | 100% activity | Pectobacterium atrosepticum | 1-pentanol + NAD(P)+ | - |
? | |
1.1.1.71 | propanal + NADPH + H+ | 14% activity compared to pentanal | Pectobacterium atrosepticum | 1-propanol + NAD(P)+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.2 | dimer | 2 * 42100, calculated, 2 * 42000, SDS-PAGE | Pectobacterium atrosepticum |
1.1.1.71 | ? | x * 42100, calculated from amino acid sequence | Pectobacterium atrosepticum |
1.1.1.71 | ? | x * 43200, SDS-PAGE | Pectobacterium atrosepticum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.2 | YqhD | - |
Pectobacterium atrosepticum |
1.1.1.71 | ADH | - |
Pectobacterium atrosepticum |
1.1.1.71 | NAD(P)+-dependent alcohol dehydrogenase | - |
Pectobacterium atrosepticum |
1.1.1.71 | YqhD | - |
Pectobacterium atrosepticum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 40 | - |
- |
Pectobacterium atrosepticum |
1.1.1.71 | 40 | - |
- |
Pectobacterium atrosepticum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 20 | 30 | 30-60% of maximum activity | Pectobacterium atrosepticum |
1.1.1.71 | 10 | 50 | 30 to 60% of activity is observed between 20° and 30°C and 20% activity at 10 and 50°C, respectively | Pectobacterium atrosepticum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 4 | 40 | stable for at least 30 min | Pectobacterium atrosepticum |
1.1.1.2 | 50 | - |
complete inactivation | Pectobacterium atrosepticum |
1.1.1.71 | 4 | 60 | the enzyme is thermally stable when incubated for 30 min between 4 and 40°C. Incubation for 30 min at temperatures between 10 and 30 °C slightly increases the catalytic activity. Recombinant enzyme is completely inactivated at 50 and 60°C | Pectobacterium atrosepticum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.2 | 20.1 | - |
Butanal | pH 7.0, temperature not specified in the publication | Pectobacterium atrosepticum | |
1.1.1.2 | 49.2 | - |
pentanal | pH 7.0, temperature not specified in the publication | Pectobacterium atrosepticum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 7 | - |
- |
Pectobacterium atrosepticum |
1.1.1.71 | 7 | - |
- |
Pectobacterium atrosepticum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.71 | 6 | 8 | the enzyme shows around 50% of activity at pH 6.0, pH 6.5, and pH 8.0 | Pectobacterium atrosepticum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.2 | NADPH | - |
Pectobacterium atrosepticum | |
1.1.1.71 | NADPH | - |
Pectobacterium atrosepticum |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.1.1.2 | Pectobacterium atrosepticum | calculated | - |
5.5 |
1.1.1.71 | Pectobacterium atrosepticum | calculated from amino acid sequence | - |
5.5 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.2 | 3 | - |
Butanal | pH 7.0, temperature not specified in the publication | Pectobacterium atrosepticum | |
1.1.1.2 | 123.1 | - |
pentanal | pH 7.0, temperature not specified in the publication | Pectobacterium atrosepticum |