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Literature summary extracted from

  • Reisse, S.; Garbe, D.; Brueck, T.
    Identification and optimization of a novel thermo- and solvent stable ketol-acid reductoisomerase for cell free isobutanol biosynthesis (2015), Biochimie, 108, 76-84.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.86 expressed in Escherichia coli Rosetta (DE3) cells Meiothermus ruber

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.86 T84S the mutant shows increased catalytic efficiency compared to the wild type enzyme Meiothermus ruber

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.86 0.02
-
 NADPH wild type enzyme, at pH 7.0 and 50°C Meiothermus ruber
1.1.1.86 0.04
-
 (S)-2-acetolactate mutant enzyme T84S, with NADH as cosubstrate, at pH 7.0 and 50°C Meiothermus ruber
1.1.1.86 0.08
-
 (S)-2-acetolactate wild type enzyme, with NADPH as cosubstrate, at pH 7.0 and 50°C Meiothermus ruber
1.1.1.86 0.15
-
 (S)-2-acetolactate mutant enzyme T84S, with NADPH as cosubstrate, at pH 7.0 and 50°C Meiothermus ruber
1.1.1.86 0.24
-
 (S)-2-acetolactate wild type enzyme, with NADH as cosubstrate, at pH 7.0 and 50°C Meiothermus ruber

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.86 39000
-
-
 Meiothermus ruber
1.1.1.86 40000
-
 1 * 40000, SDS-PAGE Meiothermus ruber

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.86 Meiothermus ruber D3PT81
-
-
1.1.1.86 Meiothermus ruber DSM 1279 D3PT81
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.86 Ni-NTA column chromatography Meiothermus ruber

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.86 (S)-2-acetolactate + NADH + H+
-
 Meiothermus ruber (R)-2,3-dihydroxyisovalerate + NAD+
-
 r
1.1.1.86 (S)-2-acetolactate + NADH + H+
-
 Meiothermus ruber DSM 1279 (R)-2,3-dihydroxyisovalerate + NAD+
-
 r
1.1.1.86 (S)-2-acetolactate + NADPH + H+
-
 Meiothermus ruber (R)-2,3-dihydroxyisovalerate + NADP+
-
 r
1.1.1.86 (S)-2-acetolactate + NADPH + H+
-
 Meiothermus ruber DSM 1279 (R)-2,3-dihydroxyisovalerate + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.86 monomer 1 * 40000, SDS-PAGE Meiothermus ruber
1.1.1.86 monomer 1 * 39000, calculated from amino acid sequence Meiothermus ruber

Synonyms

EC Number Synonyms Comment Organism
1.1.1.86 KARI
-
 Meiothermus ruber

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.86 65
-
-
 Meiothermus ruber

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.86 50
-
 at 50°C, the enzyme shows an half-life of 71 h Meiothermus ruber

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.86 0.77
-
 (S)-2-acetolactate wild type enzyme, with NADPH as cosubstrate, at pH 7.0 and 50°C Meiothermus ruber
1.1.1.86 0.98
-
 NADPH wild type enzyme, at pH 7.0 and 50°C Meiothermus ruber
1.1.1.86 1.09
-
 (S)-2-acetolactate wild type enzyme, with NADH as cosubstrate, at pH 7.0 and 50°C Meiothermus ruber
1.1.1.86 1.3
-
 (S)-2-acetolactate mutant enzyme T84S, with NADH as cosubstrate, at pH 7.0 and 50°C Meiothermus ruber
1.1.1.86 3.81
-
 (S)-2-acetolactate mutant enzyme T84S, with NADPH as cosubstrate, at pH 7.0 and 50°C Meiothermus ruber

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.86 7
-
-
 Meiothermus ruber

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.86 NADH prefers NADPH over NADH Meiothermus ruber
1.1.1.86 NADPH prefers NADPH over NADH Meiothermus ruber

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.86 4.6
-
 (S)-2-acetolactate wild type enzyme, with NADH as cosubstrate, at pH 7.0 and 50°C Meiothermus ruber
1.1.1.86 10.2
-
 (S)-2-acetolactate wild type enzyme, with NADPH as cosubstrate, at pH 7.0 and 50°C Meiothermus ruber
1.1.1.86 25.9
-
 (S)-2-acetolactate mutant enzyme T84S, with NADPH as cosubstrate, at pH 7.0 and 50°C Meiothermus ruber
1.1.1.86 34.1
-
 (S)-2-acetolactate mutant enzyme T84S, with NADH as cosubstrate, at pH 7.0 and 50°C Meiothermus ruber
1.1.1.86 54.5
-
 NADPH wild type enzyme, at pH 7.0 and 50°C Meiothermus ruber