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Literature summary extracted from

  • Herrmann, J.; Nathin, D.; Lee, S.G.; Sun, T.; Jez, J.M.
    Recapitulating the structural evolution of redox regulation in adenosine 5'-phosphosulfate kinase from cyanobacteria to Plants (2015), J. Biol. Chem., 290, 24705-24714.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.25 expressed in Escherichia coli BL21(DE3) cells Synechocystis sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.25 in complex with AMP-PNP and adenosine-5'-phosphosulfate, hanging drop vapor diffusion method, using 0.1 M sodium cacodylate trihydrate, pH 6.5, 0.2 M magnesium acetate tetrahydrate, and 20% (w/v) PEG 8000 Synechocystis sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.25 H23C the mutation does not significantly alter the steady-state kinetic parameters of the enzyme Synechocystis sp.
2.7.1.25 T61E the mutation does not significantly alter the steady-state kinetic parameters of the enzyme Synechocystis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.25 adenosine-5'-phosphosulfate substrate inhibition Synechocystis sp.
2.7.1.25 sulfate
-
 Synechocystis sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.25 20000
-
 2 * 20000, SDS-PAGE Synechocystis sp.
2.7.1.25 40000
-
 gel filtration Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.25 ATP + adenosine-5'-phosphosulfate Synechocystis sp.
-
 ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Synechocystis sp. P72940
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.25 Ni-NTA column chromatography, Ni-NTA/benzamidine-Sepharose column chromatography, and Superdex 200 gel filtration Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine-5'-phosphosulfate
-
 Synechocystis sp. ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.25 homodimer 2 * 20000, SDS-PAGE Synechocystis sp.

Synonyms

EC Number Synonyms Comment Organism
2.7.1.25 adenosine 5'-phosphosulfate kinase
-
 Synechocystis sp.
2.7.1.25 APS kinase
-
 Synechocystis sp.
2.7.1.25 APSK
-
 Synechocystis sp.

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.25 15.7
-
 mutant enzyme T61E, pH and temperature not specified in the publication Synechocystis sp. sulfate
2.7.1.25 17
-
 wild type enzyme, pH and temperature not specified in the publication Synechocystis sp. sulfate