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Literature summary extracted from

  • Famiani, F.; Paoletti, A.; Battistelli, A.; Moscatello, S.; Chen, Z.H.; Leegood, R.C.; Walker, R.P.
    Phosphoenolpyruvate carboxykinase, pyruvate orthophosphate dikinase and isocitrate lyase in both tomato fruits and leaves, and in the flesh of peach and some other fruits (2016), J. Plant Physiol., 202, 34-44.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.9.1 cytoplasm
-
Zea mays 5737
-
2.7.9.1 cytoplasm
-
Solanum lycopersicum 5737
-
2.7.9.1 cytoplasm
-
Capsicum annuum 5737
-
2.7.9.1 cytoplasm
-
Hoya carnosa 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.9.1 95000
-
x * 95000, SDS-PAGE Zea mays
2.7.9.1 95000
-
x * 95000, SDS-PAGE Solanum lycopersicum
2.7.9.1 95000
-
x * 95000, SDS-PAGE Hoya carnosa

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.1 Capsicum annuum
-
cv. World Beater
-
2.7.9.1 Hoya carnosa
-
-
-
2.7.9.1 no activity in Prunus persica var. Adriatica
-
-
-
2.7.9.1 no activity in Solanum melongena var. Black Enorma
-
-
-
2.7.9.1 Solanum lycopersicum
-
cv. Moneymaker
-
2.7.9.1 Zea mays
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.9.1 phosphoprotein in the darkened leaves of C3 plants PPDK is present largely as an inactive phosphorylated form Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.9.1 fruit fruit wall of green and red pepper, low enzyme content Capsicum annuum
-
2.7.9.1 fruit ripe fruit flesh, the enzyme is located in skin, flesh, locular gel and columella of tomato fruits. Its abundance does not increase during ripening, but decreases during senescence Solanum lycopersicum
-
2.7.9.1 leaf mature leaves, high enzyme content Zea mays
-
2.7.9.1 leaf mature leaves, high enzyme content Hoya carnosa
-
2.7.9.1 leaf the content of PPDK g/fresh weight increases in tomato leaves as they mature until the mid-stage of development, and declines during their senescence Solanum lycopersicum
-
2.7.9.1 additional information PPDK polypeptide abundance and not PPDK activity is determined, because in the darkened leaves of C3 plants, PPDK is present largely as an inactive phosphorylated form Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.9.1 0.0006
-
leaf enzyme extract, pH and temperature not specified in the publication Solanum lycopersicum
2.7.9.1 0.1 0.19 leaf enzyme extract, pH and temperature not specified in the publication Zea mays

Subunits

EC Number Subunits Comment Organism
2.7.9.1 ? x * 95000, SDS-PAGE Zea mays
2.7.9.1 ? x * 95000, SDS-PAGE Solanum lycopersicum
2.7.9.1 ? x * 95000, SDS-PAGE Hoya carnosa

Synonyms

EC Number Synonyms Comment Organism
2.7.9.1 PPDK
-
Zea mays
2.7.9.1 PPDK
-
Solanum lycopersicum
2.7.9.1 PPDK
-
Capsicum annuum
2.7.9.1 PPDK
-
Hoya carnosa
2.7.9.1 pyruvate orthophosphate dikinase
-
Zea mays
2.7.9.1 pyruvate orthophosphate dikinase
-
Solanum lycopersicum
2.7.9.1 pyruvate orthophosphate dikinase
-
Capsicum annuum
2.7.9.1 pyruvate orthophosphate dikinase
-
Hoya carnosa

General Information

EC Number General Information Comment Organism
2.7.9.1 metabolism the enzyme is involved in gluconeogenesis Zea mays
2.7.9.1 metabolism the enzyme is involved in gluconeogenesis Hoya carnosa
2.7.9.1 metabolism the enzyme is involved in gluconeogenesis. In fruits from other plants than tomato, the bulk of any gluconeogenic flux proceeds via phosphoenolpyruvate carboxykinase, PEPCK Capsicum annuum
2.7.9.1 metabolism the enzyme is involved in gluconeogenesis. In fruits from other plants than tomato, the bulk of any gluconeogenic flux proceeds via phosphoenolpyruvate carboxykinase, PEPCK, whereas in tomato both PEPCK and PPDK can potentially be utilised. The conversion of pyruvate/acetyl-CoA to malate by the glyoxylate cycle, for which glyoxysomal isocitrate lyase is necessary, is not a major pathway utilised by gluconeogenesis in fruits under normal conditions of growth Solanum lycopersicum