EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.383 | biofuel production | Recent work has demonstrated glucose to isobutanol conversion through a modified amino acid pathway in a recombinant organism. We demonstrate that an NADH-dependent pathway enables anaerobic isobutanol production at 100% theoretical yield and at higher titer and productivity than both the NADPH-dependent pathway and transhydrogenase over-expressing strain | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.383 | A71S | mutant engineered to use NADH instead of NADPH | Escherichia coli |
1.1.1.383 | A71S/R76D/S78D/Q110A | mutant engineered to use NADH instead of NADPH | Escherichia coli |
1.1.1.383 | A71S/R76D/S78D/Q110V | mutant engineered to use NADH instead of NADPH | Escherichia coli |
1.1.1.383 | Q110A | mutant engineered to use NADH instead of NADPH | Escherichia coli |
1.1.1.383 | Q110V | mutant engineered to use NADH instead of NADPH | Escherichia coli |
1.1.1.383 | R76D | mutant engineered to use NADH instead of NADPH | Escherichia coli |
1.1.1.383 | S78D | mutant engineered to use NADH instead of NADPH | Escherichia coli |
1.1.1.383 | S78D/Q110V | mutant engineered to use NADH instead of NADPH | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.383 | 0.013 | - |
NADPH | mutant Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 0.024 | - |
NADPH | mutant Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 0.03 | - |
NADH | mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 0.04 | - |
NADPH | wild-type, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 0.04 | - |
NADH | mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 0.065 | - |
NADPH | mutant S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 0.065 | - |
NADH | mutant S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 0.13 | - |
NADH | mutant S78D, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 0.14 | - |
NADH | mutant Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 0.28 | - |
NADH | mutant Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 0.57 | - |
NADPH | mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 0.65 | - |
NADPH | mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 0.66 | - |
NADPH | mutant S78D, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 1.08 | - |
NADH | wild-type, pH 7.0, temperature not specified in the publication | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.383 | Escherichia coli | P05793 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.383 | (S)-2-acetolactate + NADH + H+ | - |
Escherichia coli | 2,3-dihydroxy-3-methylbutanoate + NAD+ | - |
? | |
1.1.1.383 | (S)-2-acetolactate + NADPH + H+ | - |
Escherichia coli | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.383 | 0.2 | - |
NADPH | mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 0.3 | - |
NADH | wild-type, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 0.6 | - |
NADPH | mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 2 | - |
NADH | mutant S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 2.2 | - |
NADPH | mutant S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 2.3 | - |
NADH | mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 2.5 | - |
NADPH | mutant S78D, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 2.6 | - |
NADH | mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 3.1 | - |
NADH | mutant Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 3.3 | - |
NADH | mutant Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 3.6 | - |
NADPH | wild-type, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 3.6 | - |
NADH | mutant S78D, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 7.2 | - |
NADPH | mutant Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 7.2 | - |
NADPH | mutant Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.383 | 0.3 | - |
NADH | wild-type, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 0.5 | - |
NADPH | mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 1.1 | - |
NADPH | mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 3.8 | - |
NADPH | mutant S78D, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 11 | - |
NADH | mutant Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 24.8 | - |
NADH | mutant Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 27.6 | - |
NADH | mutant S78D, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 31.8 | - |
NADH | mutant S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 34.2 | - |
NADPH | mutant S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 60.3 | - |
NADH | mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 74 | - |
NADH | mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 87.3 | - |
NADPH | wild-type, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 302 | - |
NADPH | mutant Q110A, pH 7.0, temperature not specified in the publication | Escherichia coli | |
1.1.1.383 | 569 | - |
NADPH | mutant Q110V, pH 7.0, temperature not specified in the publication | Escherichia coli |