EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.62 | gene chlG, recombinant expression of N-terminally 3xFlag-tagged Chl synthase in an CHS-enzyme-deficient Synechocystis sp. strain. The cellular level of FLAG-ChlG is lower than of the native ChlG but apparently sufficient to provide chlorophyll for maintaining normal levels of photosynthetic complexes | Synechocystis sp. PCC 6803 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.62 | additional information | construction of a yidC-Flag/DELTAyidC strain that produces near-native amounts of the C-terminally FLAG-tagged YidC and normal levels of photosynthetic complexes, a very low amount of ChlG coelutes with YidC-FLAG. The HliD-less strain of Synechocystis lacks ChlG subcomplexes and accumulates chlorophyllide. The increased pool of chlorophyllide can be the consequence of lowered ChlG activity but can also arise from a defect in chlorophyll recycling | Synechocystis sp. PCC 6803 |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.5.1.62 | chloroplast membrane | - |
Synechocystis sp. PCC 6803 | 31969 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.62 | chlorophyllide a + geranylgeranyl diphosphate | Synechocystis sp. PCC 6803 | - |
geranylgeranylchlorophyllide a + diphosphate | - |
? | |
2.5.1.62 | chlorophyllide a + phytyl diphosphate | Synechocystis sp. PCC 6803 | - |
chlorophyll a + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.62 | Synechocystis sp. PCC 6803 | Q55145 | gene chlG | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.62 | recombinant 3xFLAG-tagged enzyme from Synechocystis cells, copurification of FLAG-ChlG and YidC-FLAG, by immunaffinity chromatography and ultrafiltration | Synechocystis sp. PCC 6803 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.62 | chlorophyllide a + geranylgeranyl diphosphate | - |
Synechocystis sp. PCC 6803 | geranylgeranylchlorophyllide a + diphosphate | - |
? | |
2.5.1.62 | chlorophyllide a + phytyl diphosphate | - |
Synechocystis sp. PCC 6803 | chlorophyll a + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.62 | ChlG | - |
Synechocystis sp. PCC 6803 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.62 | 30 | - |
assay at | Synechocystis sp. PCC 6803 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.62 | metabolism | identification of a link between chlorophyll biosynthesis and the Sec/YidC-dependent cotranslational insertion of nascent photosystem polypeptides into membranes. This close physical linkage coordinates the arrival of pigments and nascent apoproteins to produce photosynthetic pigment protein complexes with minimal risk of accumulating phototoxic unbound chlorophylls | Synechocystis sp. PCC 6803 |
2.5.1.62 | physiological function | the enzymatically active complex of cyanobacterial chlorophyll synthase with the high-light-inducible protein HliD associates with the Ycf39 protein, a putative assembly factor for photosystem II, and the YidC/Alb3 insertase, there is also evidence for the presence of SecY and ribosome subunits in the complex. Protein HliD is apparently required for assembly of FLAG-tagged enzyme ChlG into larger complexes with other proteins such as Ycf39. The ChG-HliD subcomplex contains chlorophyll, chlorophyllide, and carotenoids | Synechocystis sp. PCC 6803 |